Rabbit LDHA Polyclonal Antibody | anti-LDHA antibody

LDHA Antibody (CT)

Cat. Num. AAA840153

• Gene Names: LDHA; LDH1; LDHM; GSD11; PIG19; HEL-S-133P
• Reactivity: Human. Predicted cross reactivity with Monkey samples.
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: LDHA; Polyclonal Antibody; LDHA Antibody (CT); L-lactate dehydrogenase A chain; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; Renal carcinoma antigen NY-REN-59.; anti-LDHA antibody

• Host: Rabbit
• Reactivity: Human. Predicted cross reactivity with Monkey samples.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: 100 ul of antibody in PBS with 0.09% (W/V) sodium azide; Appearance: Colorless liquid
• Sequence Length: 274

• Applicable Applications for anti-LDHA antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blot: ~1:1000, IHC: ~1:10-1:50, IF: ~1:10-1:50.
• Immunogen: This LDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the C-terminal region of human LDHA.
• Positive Control: Western blot: MDA-MB231 cell lysate, IHC: human hepatocarcinoma, IF: A375 cells
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-LDHA antibody

Polyclonal Antibody to detect LDHA in human samples

Background: L-Lactate dehydrogenase A chain (LDHA) is a member of the LDH/MDH superfamily and LDH family. It catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA is localized primarily in muscle tissue and is part of the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. LDH1 is decreased in essential thrombocythemia. LDHA is induced through a non-genomic pathway of estrogen action. Reduction in LDH-A activity results in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential. Mutations in LDHA have been associated with in LDHA are the cause of glycogen storage disease type 11 (GSD11) which is a metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.

Product Categories/Family for anti-LDHA antibody

Antibodies & Supporting Tools; Antibodies for Metabolic Processes (NEW) Diabetes; Obesity & Metabolic Syndrome; Antibodies (NEW) Antibodies & Supporting Tools; Primary Antibodies (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 207028494
• NCBI GeneID: 3939
• NCBI Accession #: NP_001128711.1
• NCBI GenBank Nucleotide #: NM_001135239.1
• UniProt Accession #: P00338; Q53G53; Q6IBM7; Q6ZNV1; Q9UDE8; Q9UDE9; B4DKQ2; B7Z5E3; D3DQY3; F8W819
• Molecular Weight: 26,712 Da
• NCBI Official Full Name: L-lactate dehydrogenase A chain isoform 2
• NCBI Official Synonym Full Names: lactate dehydrogenase A
• NCBI Official Symbol: LDHA
• NCBI Official Synonym Symbols: LDH1; LDHM; GSD11; PIG19; HEL-S-133P
• NCBI Protein Information: L-lactate dehydrogenase A chain
• UniProt Protein Name: L-lactate dehydrogenase A chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: LDHA
• UniProt Synonym Gene Names: LDH-A; LDH-M
• UniProt Entry Name: LDHA_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - propanoate; EC 1.1.1.27; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Amino Acid Metabolism - cysteine and methionine; Oxidoreductase; Carbohydrate Metabolism - pyruvate; Apoptosis

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: membrane; mitochondrion; nucleus; cytosol

Molecular Function: protein binding; L-lactate dehydrogenase activity

Biological Process: cellular metabolic process; substantia nigra development; glycolysis; cellular response to extracellular stimulus; cellular carbohydrate metabolic process; pyruvate metabolic process

Disease: Glycogen Storage Disease Xi