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Western Blot (WB) (Western blot analysis of extracts from HeLa cells using 5HT5A antibody)

Rabbit 5HT5A Polyclonal Antibody | anti-5HT5A antibody

5HT5A antibody

Gene Names
Htr5a; MR22; 5HT5A
Applications
Western Blot, ELISA
Synonyms
5HT5A; Polyclonal Antibody; 5HT5A antibody; Polyclonal 5HT5A; Anti-5HT5A; HTR5A; 5-HT5A; 5-Hydroxytryptamine (serotonin) receptor 5A; anti-5HT5A antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
5HT5A antibody detects endogenous levels of total 5-HT-5A protein
Form/Format
Purified IgG supplied in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
357
Applicable Applications for anti-5HT5A antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:20000
Biological Significance
5HT5A is a member of 5-hydroxytryptamine receptor family and encodes a multi-pass membrane protein that functions as a receptor for 5-hydroxytryptamine and couples to G proteins, negatively influencing cAMP levels via Gi and Go.
Immunogen
5HT5A antibody was raised in Rabbit using synthesized peptide derived from internal of human 5HT5A as the immunogen
Preparation and Storage
Store in small aliquots at -20 degree C. Avoid repeated freeze/thaw cycles

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells using 5HT5A antibody)

Western Blot (WB) (Western blot analysis of extracts from HeLa cells using 5HT5A antibody)
Related Product Information for anti-5HT5A antibody
Rabbit polyclonal 5HT5A antibody
Product Categories/Family for anti-5HT5A antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,673 Da
NCBI Official Full Name
5-hydroxytryptamine receptor 5A
NCBI Official Synonym Full Names
5-hydroxytryptamine (serotonin) receptor 5A, G protein-coupled
NCBI Official Symbol
Htr5a
NCBI Official Synonym Symbols
MR22; 5HT5A
NCBI Protein Information
5-hydroxytryptamine receptor 5A
UniProt Protein Name
5-hydroxytryptamine receptor 5A
UniProt Gene Name
Htr5a
UniProt Synonym Gene Names
5ht5a; 5-HT-5A; 5-HT5A
UniProt Entry Name
5HT5A_RAT

NCBI Description

receptor for serotonin; involved in mediating serotonergic activity in the brain [RGD, Feb 2006]

Uniprot Description

5-HT(5A): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR

Cellular Component: Golgi apparatus; rough endoplasmic reticulum; integral to plasma membrane; dendrite; perikaryon; intracellular

Molecular Function: serotonin receptor activity

Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; cAMP-mediated signaling; hippocampus development; brain development; response to estradiol stimulus; serotonin receptor signaling pathway

Research Articles on 5HT5A

Similar Products

Product Notes

The 5HT5A htr5a (Catalog #AAA839191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 5HT5A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:20000. Researchers should empirically determine the suitability of the 5HT5A htr5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5HT5A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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