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Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells using 5HT4 antibody)

Rabbit 5HT4 Polyclonal Antibody | anti-5HT4 antibody

5HT4 antibody

Gene Names
Htr4; 5-HT4; 5HTR4; 5-HT-4; 5-HT<4L>
Applications
Western Blot, Immunofluorescence, ELISA
Synonyms
5HT4; Polyclonal Antibody; 5HT4 antibody; Polyclonal 5HT4; Anti-5HT4; HTR4; 5-HT4; 5-HT4R; 5-Hydroxytryptamine receptor 4; anti-5HT4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
5HT4 antibody detects endogenous levels of total 5-HT-4 protein
Form/Format
Purified IgG supplied in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
388
Applicable Applications for anti-5HT4 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF: 1:100-1:500
ELISA: 1:5000
Biological Significance
5HT4 is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters.
Cross-Reactivity
Mouse, Rat
Immunogen
5HT4 antibody was raised in Rabbit using synthesized peptide derived from internal of human 5HT4 as the immunogen
Preparation and Storage
Store in small aliquots at -20 degree C. Avoid repeated freeze/thaw cycles

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells using 5HT4 antibody)

Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells using 5HT4 antibody)
Related Product Information for anti-5HT4 antibody
Rabbit polyclonal 5HT4 antibody
Product Categories/Family for anti-5HT4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,312 Da
NCBI Official Full Name
5-hydroxytryptamine receptor 4
NCBI Official Synonym Full Names
5 hydroxytryptamine (serotonin) receptor 4
NCBI Official Symbol
Htr4
NCBI Official Synonym Symbols
5-HT4; 5HTR4; 5-HT-4; 5-HT<4L>
NCBI Protein Information
5-hydroxytryptamine receptor 4
UniProt Protein Name
5-hydroxytryptamine receptor 4
UniProt Gene Name
Htr4
UniProt Synonym Gene Names
5-HT-4; 5-HT4
UniProt Entry Name
5HT4R_MOUSE

Uniprot Description

5-HT(4): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. Belongs to the G-protein coupled receptor 1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass

Cellular Component: membrane; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane; endosome

Molecular Function: G-protein coupled receptor activity; protein binding; signal transducer activity; serotonin receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; synaptic transmission; regulation of appetite; positive regulation of cell proliferation; signal transduction; G-protein signaling, adenylate cyclase activating pathway; serotonin receptor signaling pathway; gamma-aminobutyric acid signaling pathway

Research Articles on 5HT4

Similar Products

Product Notes

The 5HT4 htr4 (Catalog #AAA5300365) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 5HT4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:1000 IF: 1:100-1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the 5HT4 htr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5HT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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