Rabbit MEKK2 Polyclonal Antibody | anti-MAP3K2 antibody

Anti-MEKK2 antibody

Cat. Num. AAA175490

• Gene Names: MAP3K2; MEKK2; MEKK2B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: MEKK2; Polyclonal Antibody; Anti-MEKK2 antibody; Mitogen-activated protein kinase kinase kinase 2; mitogen-activated protein kinase kinase kinase 2; M3K2_HUMAN antibody; Map3k2 antibody; MAPK/ERK kinase kinase 2 antibody; MEK kinase 2 antibody; MEKK 2 antibody; MEKK2b antibody; Mitogen activated protein kinase kinase kinase 2 antibody; Mitogen-activated protein kinase kinase kinase 2 antibody; anti-MAP3K2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 619

• Applicable Applications for anti-MAP3K2 antibody: Western Blot (WB)
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MEKK2 (606-619aa SADELLRHMFVHYH), different from the related rat and mouse sequences by one amino acid.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-MEKK2 antibody, MBS175490, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Testis Tissue LysateLane 3: Rat Lung Tissue Lysate )

Related Product Information for anti-MAP3K2 antibody

Description: Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 2(MAP3K2) detection. Tested with WB in Human, Mouse, Rat.
Background: MAP3K2, mitogen-activated protein kinase kinase kinase 2, also known as MEKK2, belongs to the mitogen-activated protein kinase(MAPK) kinase kinase gene family, which is involved in regulating multiple MAPK signaling pathways. Endogenous MAP3K2 localized mainly to the cytosol of unstimulated HeLa cells and rat fibroblasts. The MAP3K2 gene is mapped to chromosome 2 by the International Radiation Hybrid Mapping Consortium. MAP3K2 is a major upstream activator of the JNK, MAPK cascade. MAP3K2 was identifies as 1 of 6 kinases that regulate different steps of the caveolar cycle. Silencing of MAP3K2 strongly reduced kiss-and-run dynamics, leading to an accumulation of caveolar structures at the cell surface without affecting caveolae coat assembly or clustering.

NCBI and Uniprot Product Information

• NCBI GI #: 97536681
• NCBI GeneID: 10746
• UniProt Accession #: Q9Y2U5; Q53QL9; Q53S75; Q59GZ6; Q8NC32; Q9NYK3; B9EG87
• Molecular Weight: 69,741 Da
• NCBI Official Full Name: Mitogen-activated protein kinase kinase kinase 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 2
• NCBI Official Symbol: MAP3K2
• NCBI Official Synonym Symbols: MEKK2; MEKK2B
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 2; MEKK 2; MEK kinase 2; MAP/ERK kinase kinase 2; MAPK/ERK kinase kinase 2
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 2
• UniProt Gene Name: MAP3K2
• UniProt Synonym Gene Names: MAPKKK2; MEKK2; MEK kinase 2; MEKK 2
• UniProt Entry Name: M3K2_HUMAN

NCBI Description

The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process. [provided by RefSeq, Jul 2008]

Uniprot Description

MEKK2: a protein kinase of the STE11 family. Phosphorylates and activates MEK5. Involved in growth factor stimulated cell proliferation and differentiation.

Protein type: Kinase, protein; EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; STE group; STE11 family

Chromosomal Location of Human Ortholog: 2q14.3

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; metal ion binding; protein kinase binding; ATP binding; protein kinase activity

Biological Process: activation of MAPKK activity; activation of MAPK activity; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; activation of JNK activity