Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control (blue histogram) or Anti-Human CD85j (ILT) (purple histogram). Cells in the monocyte gate were used for analysis.)
Mouse CD85j Monoclonal Antibody | anti-CD85j antibody
CD85j antibody (allophycocyanin)
Applications
Flow Cytometry, Functional Assay
Synonyms
CD85j; Monoclonal Antibody; CD85j antibody (allophycocyanin); Monoclonal CD85j; Anti-CD85j; LIR1; CD 85j; CD-85j; CD-85jantibody; CD85j (APC); LILRB1; MIR7; anti-CD85j antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
HP-F1
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
5 ul (0.03 ug)/test (varies by lot)
Applicable Applications for anti-CD85j antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: 5 ul (0.03 ug)/test
A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Biological Significance
The monoclonal antibody HP-F1 recognizes CD85j, also known as ILT2, LILRB1, and LIR1. CD85j is a member of the ILT /LIR /MIR family. CD85j is a single transmembrane glycoprotein with a long cytoplasmic domain containing 4 ITIMs which signal through interactions with SHP-1. Expression is found on myeloid cells and some lymphoid cells including, subsets of NK, T and B cells. Expression has been correlated with leukemias such as ALL and CLL. Expression on CD8+ cells correlates with effector cell function and plays an important role in viral infections, including HIV, Ebstein Barr and CMV. The ligands for CD85j are MHC Class I molecules such as HLA-G, A, F, B27, E and F. The monoclonal antibody HP-F1 has been shown to reduce the amount of CD16- dependent cytolytic activity of functional NK cells.
Tag/Conjugate
Allophycocyanin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control (blue histogram) or Anti-Human CD85j (ILT) (purple histogram). Cells in the monocyte gate were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control (blue histogram) or Anti-Human CD85j (ILT) (purple histogram). Cells in the monocyte gate were used for analysis.)
Related Product Information for anti-CD85j antibody
Mouse monoclonal CD85j antibody (allophycocyanin)
Product Categories/Family for anti-CD85j antibody
Similar Products
Product Notes
The CD85j (Catalog #AAA834735) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD85j can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: 5 ul (0.03 ug)/test A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Researchers should empirically determine the suitability of the CD85j for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD85j, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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