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Flow Cytometry (FC/FACS) (Intracellular staining of C57BL/6 splenocytes with CD45R antibody and 0.25 ug of Mouse IgG1 K Isotype Control (FITC) (left) or 0.5 ug of CD79a antibody (FITC) (right). Total viable cells were used for analysis.)

Mouse CD79a Monoclonal Antibody | anti-CD79a antibody

CD79a antibody (FITC)

Gene Names
CD79A; IGA; MB-1
Applications
Flow Cytometry, Functional Assay
Synonyms
CD79a; Monoclonal Antibody; CD79a antibody (FITC); Monoclonal CD79a; Anti-CD79a; Ly-54; CD-79a; mb-1; Ig- alpha; CD 79a; anti-CD79a antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
24C2.5
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
5 ul (0.125 ug)/test (varies by lot)
Sequence Length
188
Applicable Applications for anti-CD79a antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: 5 ul (0.125 ug)/test

A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Biological Significance
This 24C2.5 monoclonal antibody reacts with the intracellular tail of mouse CD79a, a signaling component of the B cell receptor. Cd79a heterodimerizes with CD79b, and together with surface Ig, make up the BCR. Both CD79 subunits consist of a single extracellular Ig domain, a transmembrane domain, and an intracellular signaling domain. CD79a is expressed almost exclusively on B cells, as well as in most B lineage acute lymphoblastic leukemias. The cytoplasmic domain of CD79a contains immunoreceptor tyrosine-based activation motifs, which constitute the signal transducing portions of the BCR. Tyrosine phosphorylation of these ITAMs by Syk and Lyn initiates numerous signaling cascades, resulting in B cell activation, proliferation, and differentiation.
Tag/Conjugate
FITC
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.

Flow Cytometry (FC/FACS)

(Intracellular staining of C57BL/6 splenocytes with CD45R antibody and 0.25 ug of Mouse IgG1 K Isotype Control (FITC) (left) or 0.5 ug of CD79a antibody (FITC) (right). Total viable cells were used for analysis.)

Flow Cytometry (FC/FACS) (Intracellular staining of C57BL/6 splenocytes with CD45R antibody and 0.25 ug of Mouse IgG1 K Isotype Control (FITC) (left) or 0.5 ug of CD79a antibody (FITC) (right). Total viable cells were used for analysis.)
Related Product Information for anti-CD79a antibody
Mouse monoclonal CD79a antibody (FITC)
Product Categories/Family for anti-CD79a antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
973
UniProt Accession #
Molecular Weight
20,786 Da
NCBI Official Full Name
CD79a, partial
NCBI Official Synonym Full Names
CD79a molecule, immunoglobulin-associated alpha
NCBI Official Symbol
CD79A
NCBI Official Synonym Symbols
IGA; MB-1
NCBI Protein Information
B-cell antigen receptor complex-associated protein alpha chain
UniProt Protein Name
B-cell antigen receptor complex-associated protein alpha chain
UniProt Gene Name
CD79A
UniProt Synonym Gene Names
IGA; MB1
UniProt Entry Name
CD79A_HUMAN

NCBI Description

The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Ig-alpha: an integral membrane protein that associates with surface immunoglobulin B lymphocyte antigen receptor multimeric complex. Surface Ig non-covalently associates with 2 other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. Two alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: multivesicular body; integral to membrane; plasma membrane; B cell receptor complex; lipid raft; external side of plasma membrane

Molecular Function: transmembrane receptor activity

Biological Process: B cell proliferation; B cell receptor signaling pathway; B cell activation; B cell differentiation

Disease: Agammaglobulinemia 3, Autosomal Recessive

Research Articles on CD79a

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Product Notes

The CD79a cd79a (Catalog #AAA834685) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD79a can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: 5 ul (0.125 ug)/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Researchers should empirically determine the suitability of the CD79a cd79a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD79a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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