Mouse anti-Human CD79a Monoclonal Antibody | anti-CD79a antibody

anti-human CD79a-Biotin

Cat. Num. AAA666219

• Gene Names: CD79A; IGA; MB-1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD79a; Monoclonal Antibody; anti-human CD79a-Biotin; Monoclonal anti-human CD79a(mb1)/Biotin; anti-CD79a antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: ZL7-4
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 188

• Applicable Applications for anti-CD79a antibody: Flow Cytometry (FC/FACS)
• Performance: Five x 105 cultured human Daudi cells were pre incubated 5 minutes with 20 mul of 250 mug/ml human Ig (to block non specific binding) after which they were incubated 45 minutes on ice with 80 mul of anti-CD79a/Biotin at 5 mug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.42 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml anti-CD79a antibody.
• Conjugation: Biotin
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Daudi IgM complex
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/thawing not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD79a antibody

Information: Human CD79a is a 22kd glycoprotein which associates with CD79b to form a functional heterodimer that interacts with membrane immunoglobulin (mIg).The mIgM component binds antigen and the heterodimer transduces signals. Antibody ZL7-4 recognizes the extracellular part of CD79a.

References

1) L. Zhang, M.J. Glennie, et al. (1995) Therapeutic Immunol. 2: 191-202. 2) Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 180-185. 3) Chu PG, Arber DA. (2001) Appl Immunohistochem Molecul Morphol 9: 97-106.

NCBI and Uniprot Product Information

• NCBI GI #: 1087007
• NCBI GeneID: 973
• Molecular Weight: 20,786 Da
• NCBI Official Full Name: CD79a, partial
• NCBI Official Synonym Full Names: CD79a molecule, immunoglobulin-associated alpha
• NCBI Official Symbol: CD79A
• NCBI Official Synonym Symbols: IGA; MB-1
• NCBI Protein Information: B-cell antigen receptor complex-associated protein alpha chain; ig-alpha; MB-1 membrane glycoprotein; surface IgM-associated protein; CD79a antigen (immunoglobulin-associated alpha); membrane-bound immunoglobulin-associated protein
• UniProt Protein Name: B-cell antigen receptor complex-associated protein alpha chain
• Protein Family: B-cell antigen receptor complex-associated protein
• UniProt Gene Name: CD79A
• UniProt Synonym Gene Names: IGA; MB1
• UniProt Entry Name: CD79A_HUMAN

NCBI Description

The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Ig-alpha: an integral membrane protein that associates with surface immunoglobulin B lymphocyte antigen receptor multimeric complex. Surface Ig non-covalently associates with 2 other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. Two alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: multivesicular body; plasma membrane; integral to membrane; B cell receptor complex; external side of plasma membrane; lipid raft

Molecular Function: transmembrane receptor activity

Biological Process: B cell proliferation; B cell receptor signaling pathway; B cell activation; B cell differentiation

Disease: Agammaglobulinemia 3, Autosomal Recessive