Mouse PSMB7 Monoclonal Antibody | anti-PSMB7 antibody

PSMB7 antibody

Cat. Num. AAA834615

• Gene Names: PSMB7; Z
• Applications: Immunohistochemistry, Western Blot
• Purity: PSMB7 antibody was purified by affinity chromatography.
• Synonyms: PSMB7; Monoclonal Antibody; PSMB7 antibody; Monoclonal PSMB7; Anti-PSMB7; Z; PSMB 7; PSMB-7; anti-PSMB7 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1E1
• Purity/Purification: PSMB7 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5-1 mg/ml (varies by lot)
• Sequence Length: 277

• Applicable Applications for anti-PSMB7 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:150; WB: 1:500-2000
• Biological Significance: This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit.
• Cross-Reactivity: Human, Monkey, Mouse
• Immunogen: PSMB7 antibody was raised in Mouse using Human recombinant protein fragment corresponding to amino acids 58-277 of human PSMB7 produced in E Coli as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of PSMB7 protein in paraffin embedded Adenocarcinoma of Human breast tissue using PSMB7 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PSMB7 protein (Right) or empty vector (Left) detected with PSMB7 antibody)

Related Product Information for anti-PSMB7 antibody

Mouse monoclonal PSMB7 antibody

Product Categories/Family for anti-PSMB7 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 48145559
• NCBI GeneID: 5695
• UniProt Accession #: Q6IBT1; Q99436
• Molecular Weight: 29,937 Da
• NCBI Official Full Name: PSMB7
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, beta type, 7
• NCBI Official Symbol: PSMB7
• NCBI Official Synonym Symbols: Z
• NCBI Protein Information: proteasome subunit beta type-7
• UniProt Protein Name: Proteasome subunit beta type
• Protein Family: Proteasome
• UniProt Gene Name: PSMB7
• UniProt Entry Name: Q6IBT1_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. A pseudogene of this gene is located on the long arm of chromosome 14. [provided by RefSeq, Jul 2012]

Uniprot Description

PSMB7: a proteasomal protein of the T1B peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. Not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit).

Protein type: Proteasome complex; Protease; EC 3.4.25.1

Chromosomal Location of Human Ortholog: 9q34.11-q34.12

Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; cytoplasm; cytosol; nucleus

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle