Mouse PSMB9 Monoclonal Antibody | anti-PSMB9 antibody

PSMB9 antibody

Cat. Num. AAA832966

• Gene Names: PSMB9; LMP2; PSMB6i; RING12; beta1i
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
• Purity: PSMB9 antibody was purified by affinity chromatography.
• Synonyms: PSMB9; Monoclonal Antibody; PSMB9 antibody; Monoclonal PSMB9; Anti-PSMB9; beta1i; LMP2; MGC70470; PSMB6i; RING12; PSMB 9; PSMB-9; anti-PSMB9 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 1C4
• Purity/Purification: PSMB9 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5-1 mg/ml (varies by lot)
• Sequence Length: 219

• Applicable Applications for anti-PSMB9 antibody: Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: FC: 1:100; IHC: 1:150; WB: 1:2000
• Biological Significance: This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit.
• Cross-Reactivity: Human
• Immunogen: PSMB9 antibody was raised in Mouse using Human recombinant protein fragment corresponding to amino acids 21-219 of human PSMB9 produced in HEK293T cell as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HEK293T cells transfected with either recombinant PSMB9 protein (red) or empty vector (blue) stained using PSMB9 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PSMB9 protein (Right) or empty vector (Left) detected with PSMB9 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of PSMB9 protein in paraffin embedded Carcinoma of Human kidney tissue using PSMB9 antibody)

Related Product Information for anti-PSMB9 antibody

Mouse monoclonal PSMB9 antibody

Product Categories/Family for anti-PSMB9 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4506205
• NCBI GeneID: 5698
• NCBI Accession #: NP_002791.1
• NCBI GenBank Nucleotide #: NM_002800.4
• Molecular Weight: 22,328 Da
• NCBI Official Full Name: proteasome subunit beta type-9 proprotein
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, beta type, 9
• NCBI Official Symbol: PSMB9
• NCBI Official Synonym Symbols: LMP2; PSMB6i; RING12; beta1i
• NCBI Protein Information: proteasome subunit beta type-9
• UniProt Protein Name: Proteasome subunit beta type-9
• Protein Family: Proteasome
• UniProt Gene Name: PSMB9
• UniProt Synonym Gene Names: LMP2; PSMB6i; RING12
• UniProt Entry Name: PSB9_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. [provided by RefSeq, Mar 2010]

Uniprot Description

PSMB9: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Belongs to the peptidase T1B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; EC 3.4.25.1; Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; cytoplasm; cytosol

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle