Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PVRL1 protein using PVRL1 antibody)
Mouse PVRL1 Monoclonal Antibody | anti-PVRL1 antibody
PVRL1 antibody
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
PVRL1 antibody was purified by affinity chromatography.
Synonyms
PVRL1; Monoclonal Antibody; PVRL1 antibody; Monoclonal PVRL1; Anti-PVRL1; CD111; CLPED1; ED4; HIgR; HVEC; MGC142031; MGC16207; nectin-1; OFC7; PRR; PRR1; PVRR; PVRR1; SK-12; PVRL 1; PVRL-1; anti-PVRL1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6F8
Purity/Purification
PVRL1 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
0.5-1 mg/ml (varies by lot)
Applicable Applications for anti-PVRL1 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100
IHC: 1:150
WB: 1:2000
IHC: 1:150
WB: 1:2000
Biological Significance
This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P).
Cross-Reactivity
Human
Immunogen
PVRL1 antibody was raised in Mouse using full length human recombinant protein of human PVRL1 produced in HEK293T cell as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PVRL1 protein using PVRL1 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PVRL1 protein using PVRL1 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PVRL1 protein (Right) or empty vector (Left) detected with PVRL1 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PVRL1 protein (Right) or empty vector (Left) detected with PVRL1 antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PVRL1 protein in paraffin embedded Carcinoma of Human bladder tissue using PVRL1 antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PVRL1 protein in paraffin embedded Carcinoma of Human bladder tissue using PVRL1 antibody)
Related Product Information for anti-PVRL1 antibody
Mouse monoclonal PVRL1 antibody
Product Categories/Family for anti-PVRL1 antibody
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Product Notes
The PVRL1 (Catalog #AAA833216) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PVRL1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100 IHC: 1:150 WB: 1:2000. Researchers should empirically determine the suitability of the PVRL1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PVRL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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