Mouse NHEJ1 Monoclonal Antibody | anti-NHEJ1 antibody

NHEJ1 antibody

Cat. Num. AAA832760

• Gene Names: NHEJ1; XLF
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: NHEJ1 antibody was purified by affinity chromatography.
• Synonyms: NHEJ1; Monoclonal Antibody; NHEJ1 antibody; Monoclonal NHEJ1; Anti-NHEJ1; FLJ12610; XLF; NHEJ 1; NHEJ-1; anti-NHEJ1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 1F3
• Purity/Purification: NHEJ1 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5-1 mg/ml (varies by lot)
• Sequence Length: 140

• Applicable Applications for anti-NHEJ1 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:100; IHC: 1:150; WB: 1:500-2000
• Biological Significance: Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.
• Cross-Reactivity: Human
• Immunogen: NHEJ1 antibody was raised in Mouse using full length human recombinant protein of human NHEJ1 produced in HEK293T cell as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NHEJ1 protein using NHEJ1 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NHEJ1 protein (Right) or empty vector (Left) detected with NHEJ1 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of NHEJ1 protein in paraffin embedded Human Kidney tissue using NHEJ1 antibody)

Related Product Information for anti-NHEJ1 antibody

Mouse monoclonal NHEJ1 antibody

Product Categories/Family for anti-NHEJ1 antibody

DNA & RNA

NCBI and Uniprot Product Information

• NCBI GI #: 34782961
• NCBI GeneID: 79840
• UniProt Accession #: Q9H9Q4; Q4ZFW7; Q6IA64; Q96JS9; B8ZZA4
• Molecular Weight: 35,291 Da
• NCBI Official Full Name: NHEJ1 protein, partial
• NCBI Official Synonym Full Names: nonhomologous end-joining factor 1
• NCBI Official Symbol: NHEJ1
• NCBI Official Synonym Symbols: XLF
• NCBI Protein Information: non-homologous end-joining factor 1
• UniProt Protein Name: Non-homologous end-joining factor 1
• Protein Family: Non-homologous end-joining factor
• UniProt Gene Name: NHEJ1
• UniProt Synonym Gene Names: XLF
• UniProt Entry Name: NHEJ1_HUMAN

NCBI Description

Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

NHEJ1: DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary. Interacts with XRCC4 and the XRCC4-LIG4 complex. Binds DNA in a length-dependent manner. Ubiquitously expressed. Belongs to the XLF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding

Biological Process: central nervous system development; B cell differentiation; response to ionizing radiation; positive regulation of ligase activity; double-strand break repair via nonhomologous end joining; T cell differentiation; DNA recombination

Disease: Severe Combined Immunodeficiency With Microcephaly, Growth Retardation, And Sensitivity To Ionizing Radiation