Mouse IL23 Monoclonal Antibody | anti-IL23 antibody

IL23 antibody

Cat. Num. AAA832641

• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Purity: IL23 antibody was purified by affinity chromatography.
• Synonyms: IL23; Monoclonal Antibody; IL23 antibody; Monoclonal IL23; Anti-IL23; (Interleukin-23 subunit p19; IL-23; Interleukin-23 p19 Interleukin-23 subunit alpha; IL-23p19; IL 23; IL-23 subunit alpha; IL-23-A; anti-IL23 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone Number: 473P19
• Specificity: Mouse
• Purity/Purification: IL23 antibody was purified by affinity chromatography.
• Form/Format: Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
• Concentration: 500 ug/ml (varies by lot)

• Applicable Applications for anti-IL23 antibody: Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: FC: <= 0.5 ug/test; ; A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest
• Biological Significance: The 473P19 antibody reacts with the p19 subunit of human IL-23. The 473P19 antibody was generated from immunization with authentic, insect cell-expressed, recombinant human IL-23 heterodimer. The use of a p19-specific capture antibody and a p40-specific detection antibody yields a human IL-23 sandwich ELISA exquisitely specific for human IL-23. IL-12 p40 monomer and IL-12 p70 were run in the assay at 200 ng/ml with no interference or cross-reactivity observed. A panel of 20 unrelated cytokines was also run in the IL-23 ELISA at 100 ng/ml with no cross reactivity observed. The assay has been validated by specific detection of significant levels of native human IL-23 protein in supernatants from a variety of different activated dendritic cell populations. IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-g. Mouse IL-23 does induce strong proliferation of memory T cells, whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12 human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-g production by na¯ve and memory T cells, as compared to IL-12.IL-23-dependent, IL-17-producing CD4+ T cells have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells. More recently, de novo differentiation of Th-17 cells in the absence of IL-23 has been demonstrated by treatment of na¯ve CD4 cells with TGF-beta1 and IL-6.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Protect from light.

Related Product Information for anti-IL23 antibody

Mouse monoclonal IL23 antibody

Product Categories/Family for anti-IL23 antibody

Cytokines & Growth Factors