Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PIK3AP1 protein (Right) or empty vector (Left) detected with PIK3AP1 antibody)
Mouse PIK3AP1 Monoclonal Antibody | anti-PIK3AP1 antibody
PIK3AP1 antibody
Gene Names
SPATA2L; tamo; C16orf76
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
PIK3AP1 antibody was purified by affinity chromatography.
Synonyms
PIK3AP1; Monoclonal Antibody; PIK3AP1 antibody; Monoclonal PIK3AP1; Anti-PIK3AP1; Phosphoinositide-3-kinase adaptor protein 1; BCAP; B-cell adapter for phosphoinositide 3-kinase; B cell adaptor protein; PIKAP1 3; PIKAP1-3; anti-PIK3AP1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7B12
Purity/Purification
PIK3AP1 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
424
Applicable Applications for anti-PIK3AP1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
IHC: 1:150
WB: 1:2000
IF: 1:100
IHC: 1:150
WB: 1:2000
Biological Significance
PIK3AP1 is involved in the activation of phosphoinositide 3-kinase (PI3K) in B-cells and in natural killer (NK) cells. It couples B-cell antigen receptor (BCR) to PI3K activation by providing a docking site for the PI3K subunit PIK3R1, which contributes to B-cell development. It seems to have a complementary role with CD19 in PI3K activation. May be involved in the survival of mature B cells via activation of REL.
Cross-Reactivity
Human
Immunogen
PIK3AP1 antibody was raised in mouse using a full length recombinant protein of human PIK3AP1 (NP_689552) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PIK3AP1 protein (Right) or empty vector (Left) detected with PIK3AP1 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PIK3AP1 protein (Right) or empty vector (Left) detected with PIK3AP1 antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PIK3AP1 protein in paraffin embedded Human lymph node tissue using PIK3AP1 antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PIK3AP1 protein in paraffin embedded Human lymph node tissue using PIK3AP1 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PIK3AP1 protein using PIK3AP1 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PIK3AP1 protein using PIK3AP1 antibody)
Related Product Information for anti-PIK3AP1 antibody
Mouse monoclonal PIK3AP1 antibody
Product Categories/Family for anti-PIK3AP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,058 Da
NCBI Official Full Name
spermatogenesis-associated protein 2-like protein
NCBI Official Synonym Full Names
spermatogenesis associated 2-like
NCBI Official Symbol
SPATA2L
NCBI Official Synonym Symbols
tamo; C16orf76
NCBI Protein Information
spermatogenesis-associated protein 2-like protein
UniProt Protein Name
Spermatogenesis-associated protein 2-like protein
Protein Family
UniProt Gene Name
SPATA2L
UniProt Synonym Gene Names
C16orf76; SPATA2-like protein
UniProt Entry Name
SPA2L_HUMAN
Uniprot Description
SPATA2L: Belongs to the SPATA2 family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 16q24.3
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Product Notes
The PIK3AP1 spata2l (Catalog #AAA832087) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PIK3AP1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC: 1:100 IF: 1:100 IHC: 1:150 WB: 1:2000. Researchers should empirically determine the suitability of the PIK3AP1 spata2l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIK3AP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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