Mouse GAD1 Monoclonal Antibody | anti-GAD1 antibody

GAD1 antibody

Cat. Num. AAA831619

• Gene Names: GAD1; GAD; SCP; CPSQ1
• Applications: Immunohistochemistry, Western Blot
• Purity: GAD1 antibody was purified by affinity chromatography.
• Synonyms: GAD1; Monoclonal Antibody; GAD1 antibody; Monoclonal GAD1; Anti-GAD1; Glutamate Decarboxylase 1; FLJ45882; GAD; SCP; GAD 1; GAD-1; anti-GAD1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5D8
• Purity/Purification: GAD1 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 594

• Applicable Applications for anti-GAD1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50; WB: 1:1000
• Biological Significance: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-amino butyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67 kDa form and a less-frequent 25 kDa form.
• Cross-Reactivity: Human
• Immunogen: GAD1 antibody was raised in mouse using a protein expressed in 293T cell transfected with human GAD1 expression vector as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant GAD1 protein (Right) or empty vector (Left) detected with GAD1 antibody)

Related Product Information for anti-GAD1 antibody

Mouse monoclonal GAD1 antibody

Product Categories/Family for anti-GAD1 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 58331246
• NCBI GeneID: 2571
• NCBI Accession #: NP_000808.2
• NCBI GenBank Nucleotide #: NM_000817.2
• UniProt Accession #: Q8IVA8; Q99259
• Molecular Weight: 66,917 Da
• NCBI Official Full Name: glutamate decarboxylase 1 isoform GAD67
• NCBI Official Synonym Full Names: glutamate decarboxylase 1 (brain, 67kDa)
• NCBI Official Symbol: GAD1
• NCBI Official Synonym Symbols: GAD; SCP; CPSQ1
• NCBI Protein Information: glutamate decarboxylase 1
• UniProt Protein Name: Glutamate decarboxylase 1 (Brain, 67kDa)
• Protein Family: Glutamate decarboxylase
• UniProt Gene Name: GAD1
• UniProt Entry Name: Q8IVA8_HUMAN

NCBI Description

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]

Uniprot Description

GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 4.1.1.15; Lyase; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; Other Amino Acids Metabolism - taurine and hypotaurine

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: plasma membrane; vesicle membrane; intracellular

Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; protein N-terminus binding; pyridoxal phosphate binding

Biological Process: response to drug; glutamate catabolic process; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; gamma-aminobutyric acid biosynthetic process; protein-pyridoxal-5-phosphate linkage; neurotransmitter biosynthetic process

Disease: Cerebral Palsy, Spastic Quadriplegic, 1