Rabbit anti-Human C4BPB Polyclonal Antibody | anti-C4BPB antibody

C4BPB, ID (C4BPB, C4b-binding protein beta chain) (APC)

Cat. Num. AAA6279892

• Gene Names: C4BPB; C4BP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: C4BPB; Polyclonal Antibody; ID (C4BPB; C4b-binding protein beta chain) (APC); C4b-binding protein beta chain; anti-C4BPB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 252

• Applicable Applications for anti-C4BPB antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: C4BPB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-153 amino acids from the Central region of human C4BPB.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(C4BPB Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the C4BPB antibody detected the C4BPB protein (arrow).)

Western Blot (WB)

(Western blot analysis of C4BPB (arrow) using rabbit polyclonal C4BPB Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the C4BPB gene.)

Related Product Information for anti-C4BPB antibody

This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. A single, unique beta-chain encoded by this gene assembles with seven identical alpha-chains into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. C4b-binding protein has a regulatory role in the coagulation system also, mediated through the beta-chain binding of protein S, a vitamin K-dependent protein that serves as a cofactor of activated protein C. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Alternative splicing gives rise to multiple transcript variants.

Product Categories/Family for anti-C4BPB antibody

Antibodies; Binding Proteins

References

Han, S., et al. Hum. Immunol. 71(7):727-730(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Buil, A., et al. Blood 115(23):4644-4650(2010). Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 62912464
• NCBI GeneID: 725
• NCBI Accession #: NP_001017365.1; NP_001017367.1; NP_000707.1
• NCBI GenBank Nucleotide #: NP_001017365.1
• UniProt Accession #: P20851
• NCBI Official Full Name: C4b-binding protein beta chain isoform 1
• NCBI Official Synonym Full Names: complement component 4 binding protein beta
• NCBI Official Symbol: C4BPB
• NCBI Official Synonym Symbols: C4BP
• NCBI Protein Information: C4b-binding protein beta chain
• UniProt Protein Name: C4b-binding protein beta chain
• Protein Family: C4b-binding protein
• UniProt Gene Name: C4BPB
• UniProt Entry Name: C4BPB_HUMAN

NCBI Description

This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. A single, unique beta-chain encoded by this gene assembles with seven identical alpha-chains into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. C4b-binding protein has a regulatory role in the coagulation system also, mediated through the beta-chain binding of protein S, a vitamin K-dependent protein that serves as a cofactor of activated protein C. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Alternative splicing gives rise to multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

C4BPB: Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: protein binding

Biological Process: negative regulation of complement activation, classical pathway; positive regulation of protein catabolic process; regulation of complement activation; innate immune response; blood coagulation; complement activation, classical pathway