Rabbit Granzyme H Polyclonal Antibody | anti-G75 antibody

Granzyme H (G75) Polyclonal Antibody

Cat. Num. AAA3002758

• Gene Names: GZMH; CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Granzyme H; Polyclonal Antibody; Granzyme H (G75) Polyclonal Antibody; CCP-X; Cathepsin G-like 2; CTSGL2; Cytotoxic T-lymphocyte proteinase; Cytotoxic serine protease C; CSP-C; GZMH; CGL2; anti-G75 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Granzyme H (G75) polyclonal antibody detects endogenous levels of Granzyme H protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 246

• Applicable Applications for anti-G75 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human Granzyme H.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Granzyme H (G75) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing Secreted and Cytoplasmic granule staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Granzyme H (G75) pAb in paraffin-embedded human lung carcinoma tissue at 1:50.showing Secreted and Cytoplasmic granule staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-G75 antibody

The Granzyme family of proteins belong to the larger peptidase S1 family.Granzyme H, also designated cytotoxic T-lymphocyte proteinase, cathepsin G-like 2 (CTSGL2) or cytotoxic serine protease C (CSP-C), contains one peptidase S1 domain. Granzyme H localizes to cytoplasmic granules of cytolytic T-lymphocytes and is important for target cell lysis in cell-mediated immune responses.

NCBI and Uniprot Product Information

• NCBI GI #: 15529990
• NCBI GeneID: 2999
• NCBI Accession #: NP_219491.1
• NCBI GenBank Nucleotide #: NP_219491.1
• UniProt Accession #: P20718
• Molecular Weight: ~ 21, 27kDa
• NCBI Official Full Name: granzyme H isoform 1 preproprotein
• NCBI Official Synonym Full Names: granzyme H
• NCBI Official Symbol: GZMH
• NCBI Official Synonym Symbols: CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2
• NCBI Protein Information: granzyme H
• UniProt Protein Name: Granzyme H
• Protein Family: Granzyme
• UniProt Gene Name: GZMH
• UniProt Synonym Gene Names: CGL2; CTSGL2; CTSGL2; CSP-C
• UniProt Entry Name: GRAH_HUMAN

NCBI Description

This gene encodes a member of the peptidase S1 family of serine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a chymotrypsin-like protease. This protein is reported to be constitutively expressed in the NK (natural killer) cells of the immune system and may play a role in the cytotoxic arm of the innate immune response by inducing target cell death and by directly cleaving substrates in pathogen-infected cells. This gene is present in a gene cluster with another member of the granzyme subfamily on chromosome 14. [provided by RefSeq, Nov 2015]

Uniprot Description

GZMF: Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: EC 3.4.21.-; Protease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: serine-type endopeptidase activity

Biological Process: apoptosis; cytolysis; immune response; protein processing; proteolysis