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Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ENPEP protein (Right) or empty vector (Left) detected with ENPEP antibody)

Mouse ENPEP Monoclonal Antibody | anti-ENPEP antibody

ENPEP antibody

Gene Names
ENPEP; APA; CD249; gp160
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
ENPEP antibody was purified by affinity chromatography.
Synonyms
ENPEP; Monoclonal Antibody; ENPEP antibody; Monoclonal ENPEP; Anti-ENPEP; Glutamyl Aminopeptidase; Aminopeptidase A; CD249; gp160; Differentiation antigen gp160; EAP; anti-ENPEP antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1C4
Purity/Purification
ENPEP antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
957
Applicable Applications for anti-ENPEP antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
IHC: 1:150
WB: 1:500
Biological Significance
ENPEP appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells
Cross-Reactivity
Human
Immunogen
ENPEP antibody was raised in mouse using a full length recombinant protein of human ENPEP (NP_001968) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ENPEP protein (Right) or empty vector (Left) detected with ENPEP antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ENPEP protein (Right) or empty vector (Left) detected with ENPEP antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of ENPEP protein in paraffin embedded Carcinoma of Human kidney tissue using ENPEP antibody)

Immunohistochemistry (IHC) (Immunohistochemical analysis of ENPEP protein in paraffin embedded Carcinoma of Human kidney tissue using ENPEP antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ENPEP protein using ENPEP antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant ENPEP protein using ENPEP antibody)
Related Product Information for anti-ENPEP antibody
Mouse monoclonal ENPEP antibody
Product Categories/Family for anti-ENPEP antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
109,244 Da
NCBI Official Full Name
glutamyl aminopeptidase
NCBI Official Synonym Full Names
glutamyl aminopeptidase (aminopeptidase A)
NCBI Official Symbol
ENPEP
NCBI Official Synonym Symbols
APA; CD249; gp160
NCBI Protein Information
glutamyl aminopeptidase
UniProt Protein Name
Glutamyl aminopeptidase
UniProt Gene Name
ENPEP
UniProt Synonym Gene Names
EAP; AP-A
UniProt Entry Name
AMPE_HUMAN

Uniprot Description

ENPEP: Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells. Belongs to the peptidase M1 family.

Protein type: Membrane protein, integral; EC 3.4.11.7; Protease

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: integral to plasma membrane; apical part of cell; lysosomal membrane; apical plasma membrane; plasma membrane; cytoplasmic vesicle; brush border; external side of plasma membrane

Molecular Function: metallopeptidase activity; zinc ion binding; aminopeptidase activity

Biological Process: cell proliferation; cell migration; cellular protein metabolic process; cell-cell signaling; angiotensin catabolic process in blood; angiogenesis; angiotensin maturation; proteolysis; regulation of systemic arterial blood pressure by renin-angiotensin; glomerulus development

Research Articles on ENPEP

Similar Products

Product Notes

The ENPEP enpep (Catalog #AAA830298) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ENPEP can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC: 1:100 IF: 1:100 IHC: 1:150 WB: 1:500. Researchers should empirically determine the suitability of the ENPEP enpep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENPEP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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