Interleukin 32 Recombinant Protein | IL32 recombinant protein

Interleukin 32, 1-131aa, Human, His tag, E Coli

Cat. Num. AAA203306

• Gene Names: IL32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Interleukin 32; 1-131aa; Human; His tag; E Coli; Interleukin 32 isoform A; IL-32; Natural killer cells protein 4 (NK4); Tumor necrosis factor alpha-inducing factor (TAIF); Interleukin 32 isoform A IL 32alpha; IL 32beta; IL 32delta; IL 32gamma; Natural killer cells protein 4; NK 4; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; il32; IL32 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMCF PKVLSDDMKK LKARMHQAIE RFYDKMQNAE SGRGQVMSSL AELEDDFKEG YLETVAAYYE EQHPELTPLL EKERDGLRCR GNRSPVPDVE DPATEEPGES FCDKSYGAPR GDKEELTPQK CSEPQSSK
• Sequence Length: 131

• Applicable Applications for IL32 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for IL32 recombinant protein

Interleukin 32 isoform A belongs to cytokine family. This protein expression is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. It has the ability to induce proinflammatory cytokines such as TNFalpha and IL8 in THP1 cells, and activates typical cytokine signaling pathways involving NFkB and p38. IL-32 can also support osteoclast differentiation but not osteoclast activation by regulating the MAPK/ERK pathway and the actin cytoskeleton. Recombinant human Interleukin 32, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for IL32 recombinant protein

Hormones & Cytokines

References

Kim SH., et al. (2005). Immunity. 22 (1):131-42 ; Kobayashi H., et al. (2009). Int J Radiat Oncol Biol Phys. 74 (5):1573-9;

NCBI and Uniprot Product Information

• NCBI GI #: 61658634
• NCBI GeneID: 9235
• NCBI Accession #: NP_001012651
• NCBI GenBank Nucleotide #: NM_001012633.1
• UniProt Accession #: P24001; Q5VFH7; Q5VFH8; Q8WV38; Q96GK9; A6NNM0; A8MPX0; B4DJM1; B8Q191; D3DUB0; D3DUB2
• Molecular Weight: 19.1 kDa (168aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: interleukin-32 isoform A
• NCBI Official Synonym Full Names: interleukin 32
• NCBI Official Symbol: IL32
• NCBI Official Synonym Symbols: NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
• NCBI Protein Information: interleukin-32
• UniProt Protein Name: Interleukin-32
• Protein Family: Interleukin
• UniProt Gene Name: IL32
• UniProt Synonym Gene Names: NK4; TAIF; IL-32

NCBI Description

This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.