Mouse AK1 Monoclonal Antibody | anti-AK1 antibody

AK1 antibody

Cat. Num. AAA830065

• Gene Names: AK1; HTL-S-58j
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Purity: AK1 antibody was purified by affinity chromatography.
• Synonyms: AK1; Monoclonal Antibody; AK1 antibody; Monoclonal AK1; Anti-AK1; Adenylate Kinase 1; AK 1; AK-1; anti-AK1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8A1
• Purity/Purification: AK1 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 194

• Applicable Applications for anti-AK1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FC: 1:100; IF: 1:100; WB: 1:2500-5000
• Biological Significance: Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.
• Cross-Reactivity: Human, Monkey
• Immunogen: AK1 antibody was raised in mouse using a full length recombinant protein of human AK1 (NP_000467) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant AK1 protein (Right) or empty vector (Left) detected with AK1 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant AK1 protein using AK1 antibody)

Related Product Information for anti-AK1 antibody

Mouse monoclonal AK1 antibody

Product Categories/Family for anti-AK1 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4502011
• NCBI GeneID: 203
• NCBI Accession #: NP_000467
• NCBI GenBank Nucleotide #: NM_000476.2
• UniProt Accession #: P00568; Q9BVK9; Q9UQC7
• Molecular Weight: 21,635 Da
• NCBI Official Full Name: adenylate kinase isoenzyme 1
• NCBI Official Synonym Full Names: adenylate kinase 1
• NCBI Official Symbol: AK1
• NCBI Official Synonym Symbols: HTL-S-58j
• NCBI Protein Information: adenylate kinase isoenzyme 1
• UniProt Protein Name: Adenylate kinase isoenzyme 1
• Protein Family: Adenylate kinase
• UniProt Gene Name: AK1
• UniProt Synonym Gene Names: AK 1
• UniProt Entry Name: KAD1_HUMAN

NCBI Description

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme. [provided by RefSeq, Jul 2008]

Uniprot Description

AK1: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Defects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency (HAAKD). Belongs to the adenylate kinase family.

Protein type: EC 2.7.4.3; Kinase, other; Nucleotide Metabolism - purine; EC 2.7.4.6

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: cytoplasm; plasma membrane; outer dense fiber; cytosol

Molecular Function: nucleoside diphosphate kinase activity; adenylate kinase activity; ATP binding

Biological Process: ATP metabolic process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; nucleoside triphosphate biosynthetic process; ADP biosynthetic process; nucleoside diphosphate phosphorylation; AMP metabolic process; cell cycle arrest

Disease: Adenylate Kinase Deficiency, Hemolytic Anemia Due To