Mouse anti-Human, Mouse EphA4 Monoclonal Antibody | anti-EphA4 antibody

EphA4 (Ephrin Type-A Receptor 4, EPH Receptor A4, EPH-like Kinase 8, EK8, hEK8, SEK, Tyrosine-protein Kinase TYRO1, TYRO1, Tyrosine-protein Kinase Receptor SEK) (AP)

Cat. Num. AAA6131143

• Gene Names: EPHA4; SEK; HEK8; TYRO1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EphA4; Monoclonal Antibody; EphA4 (Ephrin Type-A Receptor 4; EPH Receptor A4; EPH-like Kinase 8; EK8; hEK8; SEK; Tyrosine-protein Kinase TYRO1; TYRO1; Tyrosine-protein Kinase Receptor SEK) (AP); anti-EphA4 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 6H7
• Specificity: Recognizes human EPHA4. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-EphA4 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa887-986 from human EPHA4 (NP_004429) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: SLKRTGTESSRPNTALLDPSSPEFSAVVSVGDWLQAIKMDRYKDNFTAAGYTTLEAVVHVNQEDLARIGITAITHQNKILSSVQAMRTQMQQMHGRMVPV
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (36.74kD) using 126382.)

Western Blot (WB)

(Western Blot analysis of EPHA4 expression in NIH/3T3 using 126382)

Testing Data

(Detection limit for recombinant GST tagged EPHA4 is ~0.1ng/ml as a capture antibody.)

Related Product Information for anti-EphA4 antibody

EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.

Product Categories/Family for anti-EphA4 antibody

Antibodies; Receptor Tyrosine Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4758280
• NCBI GeneID: 2043
• NCBI Accession #: NP_004429
• NCBI GenBank Nucleotide #: NM_004438
• UniProt Accession #: P54764; Q2M380; A8K2P1; B2R601; B7Z6Q8
• Molecular Weight: 104,507 Da
• NCBI Official Full Name: ephrin type-A receptor 4 isoform a
• NCBI Official Synonym Full Names: EPH receptor A4
• NCBI Official Symbol: EPHA4
• NCBI Official Synonym Symbols: SEK; HEK8; TYRO1
• NCBI Protein Information: ephrin type-A receptor 4; EK8; EPH-like kinase 8; TYRO1 protein tyrosine kinase; receptor protein-tyrosine kinase HEK8; tyrosine-protein kinase TYRO1; tyrosine-protein kinase receptor SEK
• UniProt Protein Name: Ephrin type-A receptor 4
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA4
• UniProt Synonym Gene Names: HEK8; SEK; TYRO1; EK8; hEK8
• UniProt Entry Name: EPHA4_HUMAN

Uniprot Description

EphA4: a tyrosine kinase receptor of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in hindbrain pattern formation. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels.

Protein type: EC 2.7.10.1; Kinase, protein; Protein kinase, tyrosine (receptor); Membrane protein, integral; Protein kinase, TK; TK group; Eph family

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: Golgi apparatus; cell surface; endoplasmic reticulum; integral to plasma membrane; postsynaptic density; dendrite; dendritic spine; perikaryon; postsynaptic membrane; mitochondrial outer membrane; axon; early endosome membrane; cytoplasm; plasma membrane; nerve terminal; neuromuscular junction; cell junction; filopodium

Molecular Function: identical protein binding; protein binding; ephrin receptor binding; transmembrane-ephrin receptor activity; GPI-linked ephrin receptor activity; PH domain binding; ATP binding; protein kinase activity

Biological Process: axon guidance; positive regulation of JNK activity; peptidyl-tyrosine phosphorylation; negative regulation of axon regeneration; protein amino acid autophosphorylation; motor axon guidance; regulation of astrocyte differentiation; positive regulation of dendrite morphogenesis; glial cell migration; regulation of axonogenesis; adult walking behavior; ephrin receptor signaling pathway; corticospinal tract morphogenesis; cell adhesion