PIGU sirna

PIGU siRNA (Human)

Cat. Num. AAA829357

• Gene Names: PIGU; GAB1; CDC91L1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PIGU; PIGU siRNA (Human); CDC91L1; Phosphatidylinositol glycan anchor biosynthesis class U protein; Cell division cycle protein 91-like 1; Protein CDC91-like 1; GPI transamidase component PIG-U; PIGU sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PIGU siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 435

• Applicable Applications for PIGU sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PIGU gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PIGU sirna

siRNA to inhibit PIGU expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 17998700
• NCBI GeneID: 128869
• NCBI Accession #: NP_536724.1
• NCBI GenBank Nucleotide #: NM_080476.4
• UniProt Accession #: Q9H490; Q7Z489; Q8N2F2
• Molecular Weight: 47,611 Da
• NCBI Official Full Name: phosphatidylinositol glycan anchor biosynthesis class U protein
• NCBI Official Synonym Full Names: phosphatidylinositol glycan anchor biosynthesis, class U
• NCBI Official Symbol: PIGU
• NCBI Official Synonym Symbols: GAB1; CDC91L1
• NCBI Protein Information: phosphatidylinositol glycan anchor biosynthesis class U protein
• UniProt Protein Name: Phosphatidylinositol glycan anchor biosynthesis class U protein
• Protein Family: Piguamerin
• UniProt Gene Name: PIGU
• UniProt Synonym Gene Names: CDC91L1; Protein CDC91-like 1
• UniProt Entry Name: PIGU_HUMAN

NCBI Description

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Cdc91, a predicted integral membrane protein that may function in cell division control. The protein encoded by this gene is the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

PIGU: Component of the GPI transamidase complex. May be involved in the recognition of either the GPI attachment signal or the lipid portion of GPI. Belongs to the PIGU family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: endoplasmic reticulum membrane; membrane; plasma membrane; integral to endoplasmic reticulum membrane; GPI-anchor transamidase complex

Molecular Function: GPI-anchor transamidase activity

Biological Process: regulation of JAK-STAT cascade; cellular protein metabolic process; attachment of GPI anchor to protein; GPI anchor biosynthetic process; C-terminal protein lipidation; post-translational protein modification