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Western Blot (WB) (Western blot analysis of ASB3 Antibody (N-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in A549 cell line lysate. ASB3 Antibody (N-term) (arrow) was detected using the purified Pab.)

Rabbit anti-Human, mouse ASB3 Polyclonal Antibody | anti-ASB3 antibody

ASB3 Antibody (N-term)

Reactivity
Human, mouse
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ASB3; Polyclonal Antibody; ASB3 Antibody (N-term); Ankyrin repeat and SOCS box protein 3; ASB-3; anti-ASB3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ASB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-42 amino acids from the N-terminal region of human ASB3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
15-42
Sequence Length
556
Applicable Applications for anti-ASB3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of ASB3 Antibody (N-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in A549 cell line lysate. ASB3 Antibody (N-term) (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of ASB3 Antibody (N-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in A549 cell line lysate. ASB3 Antibody (N-term) (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of ASB3 Antibody (N-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse spleen tissue lysate. ASB3 Antibody (N-term) (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of ASB3 Antibody (N-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse spleen tissue lysate. ASB3 Antibody (N-term) (arrow) was detected using the purified Pab.)
Related Product Information for anti-ASB3 antibody
The protein encoded by this gene is a member of the ankyrin repeat and SOCS box-containing (ASB) family of proteins. They contain ankyrin repeat sequence and SOCS box domain. The SOCS box serves to couple suppressor of cytokine signalling (SOCS) proteins and their binding partners with the elongin B and C complex, possibly targeting them for degradation. Multiple alternatively spliced transcript variants have been described for this gene but some of the full length sequences are not known.
Product Categories/Family for anti-ASB3 antibody
References
Yang, Q., et al. BMC Med. Genet. 8 SUPPL 1, S12 (2007) : Chung, A.S., et al. Mol. Cell. Biol. 25(11):4716-4726(2005) Kile, B.T., et al. Trends Biochem. Sci. 27(5):235-241(2002) Kile, B.T., et al. Mol. Cell. Biol. 21(18):6189-6197(2001) Kile, B.T., et al. Gene 258 (1-2), 31-41 (2000) :

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57745
NCBI Official Full Name
GPR75-ASB3 protein
NCBI Official Synonym Full Names
GPR75-ASB3 readthrough
NCBI Official Symbol
GPR75-ASB3
NCBI Protein Information
GPR75-ASB3 protein
UniProt Protein Name
Ankyrin repeat and SOCS box protein 3
UniProt Gene Name
ASB3
UniProt Synonym Gene Names
ASB-3
UniProt Entry Name
ASB3_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring GPR75 (G protein-coupled receptor 75) and ASB3 (ankyrin repeat and SOCS box containing 3) on chromosome 2. The transcript includes exons from both GPR75 and ASB3 and translation initiates in the 5' non-coding exon of GPR75. The resulting protein has a novel N-terminus but is otherwise identical to that encoded by ASB3.[provided by RefSeq, Feb 2011]

Uniprot Description

ASB3: Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes TNFRSF1B. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 2p16.2

Biological Process: protein ubiquitination

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Product Notes

The ASB3 asb3 (Catalog #AAA9202741) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 15-42. The ASB3 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ASB3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the ASB3 asb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASB3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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