CD320 sirna

CD320 siRNA (Human)

Cat. Num. AAA829157

• Gene Names: CD320; 8D6; 8D6A; TCBLR
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CD320; CD320 siRNA (Human); 8D6A; CD320 antigen; 8D6 antigen; FDC-signaling molecule 8D6; FDC-SM-8D6; Transcobalamin receptor; TCblR; CD antigen CD320; CD320 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CD320 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 240

• Applicable Applications for CD320 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CD320 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CD320 sirna

siRNA to inhibit CD320 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 259906403
• NCBI GeneID: 51293
• NCBI Accession #: NP_001159367.1
• NCBI GenBank Nucleotide #: NM_001165895.1
• UniProt Accession #: Q9NPF0; Q53HF7; B2RDS5; D6W668; F5H6D3
• Molecular Weight: 24,371 Da
• NCBI Official Full Name: CD320 antigen isoform 2
• NCBI Official Synonym Full Names: CD320 molecule
• NCBI Official Symbol: CD320
• NCBI Official Synonym Symbols: 8D6; 8D6A; TCBLR
• NCBI Protein Information: CD320 antigen
• UniProt Protein Name: CD320 antigen
• Protein Family: CD320 antigen
• UniProt Gene Name: CD320
• UniProt Synonym Gene Names: 8D6A; FDC-SM-8D6; TCblR
• UniProt Entry Name: CD320_HUMAN

NCBI Description

This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin (vitamin B12), and is involved in B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011]

Uniprot Description

CD320: Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10. Receptor for the cellular uptake of transcobalamin bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC); also called methylmalonic aciduria due to transcobalamin receptor defect. MMATC is a metabolic disorder characterized by increased blood C3- acylcarnitine levels, elevated methylmalonate and homocysteine, and low uptake of transcobalamin-bound cobalamin, but normal conversion to adenosylcobalamin and methylcobalamin. Plasma vitamin B12 and total homocysteine are normal.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: membrane; endoplasmic reticulum; plasma membrane; integral to membrane; endosome membrane

Molecular Function: growth factor activity; cobalamin binding

Biological Process: vitamin metabolic process; cobalamin metabolic process; regulation of vitamin metabolic process; regulation of cell growth; water-soluble vitamin metabolic process

Disease: Methylmalonic Aciduria Due To Transcobalamin Receptor Defect