NSMAF sirna

NSMAF siRNA (Human)

Cat. Num. AAA829041

• Gene Names: NSMAF; FAN
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NSMAF; NSMAF siRNA (Human); FAN; Protein FAN; Factor associated with neutral sphingomyelinase activation; Factor associated with N-SMase activation; NSMAF sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NSMAF siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 948

• Applicable Applications for NSMAF sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NSMAF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NSMAF sirna

siRNA to inhibit NSMAF expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 221625547
• NCBI GeneID: 8439
• NCBI Accession #: NP_001138244.1
• NCBI GenBank Nucleotide #: NM_001144772.1
• UniProt Accession #: Q92636; Q8IW26; B4DFB0; E9PCH0
• Molecular Weight: 107,314 Da
• NCBI Official Full Name: protein FAN isoform 2
• NCBI Official Synonym Full Names: neutral sphingomyelinase (N-SMase) activation associated factor
• NCBI Official Symbol: NSMAF
• NCBI Official Synonym Symbols: FAN
• NCBI Protein Information: protein FAN
• UniProt Protein Name: Protein FAN
• Protein Family: Protein
• UniProt Gene Name: NSMAF
• UniProt Synonym Gene Names: FAN; Factor associated with N-SMase activation
• UniProt Entry Name: FAN_HUMAN

NCBI Description

This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]

Uniprot Description

NSMAF: Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE). Specifically binds to the N- smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Autophagy

Chromosomal Location of Human Ortholog: 8q12-q13

Cellular Component: extrinsic to membrane; cytoplasm; endomembrane system

Molecular Function: sphingomyelin phosphodiesterase activator activity; protein binding; receptor signaling protein activity; phospholipid binding

Biological Process: positive regulation of catalytic activity; positive regulation of apoptosis; signal transduction; endosome transport; ceramide metabolic process