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Western Blot (WB) (GHRL monoclonal antibody (M05), clone 2E2. Western Blot analysis of GHRL expression in HeLa.)

Mouse GHRL Monoclonal Antibody | anti-GHRL antibody

GHRL (Ghrelin/obestatin Prepropeptide, MTLRP, obestatin) (HRP)

Gene Names
GHRL; MTLRP
Applications
Western Blot
Purity
Purified
Synonyms
GHRL; Monoclonal Antibody; GHRL (Ghrelin/obestatin Prepropeptide; MTLRP; obestatin) (HRP); Ghrelin/obestatin Prepropeptide; obestatin; anti-GHRL antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
200
Specificity
Recognizes GHRL.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Sequence Length
117
Applicable Applications for anti-GHRL antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
GHRL (AAH25791.1, 1aa-117aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDEMEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK
Conjugate
HRP
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GHRL monoclonal antibody (M05), clone 2E2. Western Blot analysis of GHRL expression in HeLa.)

Western Blot (WB) (GHRL monoclonal antibody (M05), clone 2E2. Western Blot analysis of GHRL expression in HeLa.)

Testing Data

(Detection limit for recombinant GST tagged GHRL is approximately 0.1ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged GHRL is approximately 0.1ng/ml as a capture antibody.)
Product Categories/Family for anti-GHRL antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Official Full Name
Ghrelin/obestatin prepropeptide
NCBI Official Synonym Full Names
ghrelin and obestatin prepropeptide
NCBI Official Symbol
GHRL
NCBI Official Synonym Symbols
MTLRP
NCBI Protein Information
appetite-regulating hormone
Protein Family

NCBI Description

This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]

Research Articles on GHRL

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Product Notes

The GHRL (Catalog #AAA6179549) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GHRL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GHRL for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GHRL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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