KIF22 sirna

KIF22 siRNA (Human)

Cat. Num. AAA826932

• Gene Names: KIF22; KID; OBP; KNSL4; OBP-1; OBP-2; SEMDJL2; A-328A3.2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KIF22; KIF22 siRNA (Human); KID; KNSL4; Kinesin-like protein KIF22; Kinesin-like DNA-binding protein; Kinesin-like protein 4; KIF22 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KIF22 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 597

• Applicable Applications for KIF22 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KIF22 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KIF22 sirna

siRNA to inhibit KIF22 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 372266146
• NCBI GeneID: 3835
• NCBI Accession #: NP_001243198.1
• NCBI GenBank Nucleotide #: NM_001256269.1
• UniProt Accession #: Q14807; O60845; O94814; Q53F58; Q9BT46; B2R5M0; B7Z265
• Molecular Weight: 66,307 Da
• NCBI Official Full Name: kinesin-like protein KIF22 isoform 2
• NCBI Official Synonym Full Names: kinesin family member 22
• NCBI Official Symbol: KIF22
• NCBI Official Synonym Symbols: KID; OBP; KNSL4; OBP-1; OBP-2; SEMDJL2; A-328A3.2
• NCBI Protein Information: kinesin-like protein KIF22
• UniProt Protein Name: Kinesin-like protein KIF22
• Protein Family: Kinesin-like protein
• UniProt Gene Name: KIF22
• UniProt Synonym Gene Names: KID; KNSL4
• UniProt Entry Name: KIF22_HUMAN

NCBI Description

The protein encoded by this gene is a member of the kinesin-like protein family. The family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

KIF22: Kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA. Defects in KIF22 are the cause of spondyloepimetaphyseal dysplasia with joint laxity, type 2 (SEMDJL2). A bone disease characterized by short stature, distinctive midface retrusion, progressive knee malalignment (genu valgum and/or varum), generalized ligamentous laxity, and mild spinal deformity. Intellectual development is not impaired. Radiographic characteristics include significantly retarded epiphyseal ossification that evolves into epiphyseal dysplasia and precocious osteoarthritis, metaphyseal irregularities and vertical striations, constricted femoral neck, slender metacarpals and metatarsals, and mild thoracolumbar kyphosis or scoliosis with normal or mild platyspondyly. The most distinctive features for differential diagnosis of SEMDJL2 are the slender metacarpals and phalanges and the progressive degeneration of carpal bones; however, these 2 features are evident only in older children and young adults. The soft consistency of cartilage in the airways leads to laryngotracheomalacia with proneness to respiratory obstruction and inspiratory stridor in infancy and childhood. Belongs to the kinesin-like protein family.

Protein type: Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: kinetochore; nucleoplasm; microtubule; kinesin complex; focal adhesion; cytoplasm; spindle; chromatin; nucleus; cytosol

Molecular Function: DNA binding; microtubule binding; ATPase activity; microtubule motor activity; ATP binding

Biological Process: mitosis; sister chromatid cohesion; antigen processing and presentation of exogenous peptide antigen via MHC class II; DNA repair; blood coagulation; mitotic metaphase plate congression; microtubule-based movement

Disease: Spondyloepimetaphyseal Dysplasia With Joint Laxity Type 2