Rabbit MKP-2 Polyclonal Antibody | anti-MKP-2 antibody

MKP-2 (Y108) Polyclonal Antibody

Cat. Num. AAA3002441

• Gene Names: DUSP4; TYP; HVH2; MKP2; MKP-2
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MKP-2; Polyclonal Antibody; MKP-2 (Y108) Polyclonal Antibody; Dual specificity protein phosphatase 4; Dual specificity protein phosphatase hVH2; Mitogen-activated protein kinase phosphatase 2; MAP kinase phosphatase 2; DUSP4; MKP2; VH2; anti-MKP-2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MKP-2 (Y108) polyclonal antibody detects endogenous levels of MKP-2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 394

• Applicable Applications for anti-MKP-2 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 80-130 of Human MKP-2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of MKP-2 (Y108) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-MKP-2 antibody

Dual specificity phosphatases (DSPs) are a subclass of the protein tyrosine phosphatase (PTP) gene superfamily, which are selective for dephosphorylating critical phosphothreonine and phosphotyrosine residues within MAP kinases. DSP gene expression is induced by a host of growth factors and/or cellular stresses, thereby negatively regulating MAP kinase superfamily members including MAPK/ERK, SAPK/JNK and p38. The members of the dual-specificity phosphatase protein family include MKP-1/CL100 (3CH134), VHR, PAC1, MKP-2, hVH-3 (B23), hVH-5, MKP-3, MKP-X, and MKP- 4. Human MKP-2 maps to chromosome 8p12-p11 and encodes a 394 amino acid, 43 kDa protein that specifically inactivates ERK1, ERK2 and JNK.

NCBI and Uniprot Product Information

• NCBI GI #: 2499745
• NCBI GeneID: 1846
• UniProt Accession #: Q13115
• Molecular Weight: ~ 44kDa
• NCBI Official Full Name: Dual specificity protein phosphatase 4
• NCBI Official Synonym Full Names: dual specificity phosphatase 4
• NCBI Official Symbol: DUSP4
• NCBI Official Synonym Symbols: TYP; HVH2; MKP2; MKP-2
• NCBI Protein Information: dual specificity protein phosphatase 4
• UniProt Protein Name: Dual specificity protein phosphatase 4
• UniProt Gene Name: DUSP4
• UniProt Synonym Gene Names: MKP2; VH2; MAP kinase phosphatase 2; MKP-2
• UniProt Entry Name: DUS4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

MKP-2: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP4 inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Induced by hepatocyte growth factor (HGF) and vascular endothelial cell growth factor (VEGF) in human endothelial cells. Two alternatively spliced isoforms have been described. In addition, multiple polyadenylation sites have been reported. Contains 1 rhodanese domain.

Protein type: Protein phosphatase, dual-specificity; EC 3.1.3.48; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 8p12-p11

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein tyrosine/threonine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process: nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; MAPKKK cascade; toll-like receptor 3 signaling pathway; protein amino acid dephosphorylation; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; endoderm formation; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; inactivation of MAPK activity