Human Relaxin 3 ELISA Kit | RLN3 elisa kit

Human Relaxin 3 ELISA Kit

Cat. Num. AAA8248392

• Gene Names: RLN3; H3; RXN3; ZINS4; insl7
• Reactivity: Human
• Synonyms: Relaxin 3; Human Relaxin 3 ELISA Kit; INSL7; RXN3; ZINS4; Relaxin-3; Insulin-like peptide INSL7; Insulin-like peptide 7; Prorelaxin H3; RLN3 elisa kit

• Reactivity: Human
• Specificity: The Human Relaxin 3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Relaxin 3 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
• Sequence Length: 109

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RLN3 elisa kit

Principle of the Assay: The Human Relaxin 3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Relaxin 3 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human Relaxin 3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Relaxin 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Relaxin 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Relaxin 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Relaxin-3 is a neuropeptide which is highly conserved in species ranging from flies, fish, rodents and humans. It is a member and ancestral gene of the relaxin family of peptides, which includes the namesake hormone relaxin (designated 'H2 relaxin' in humans) which mediates peripheral actions during pregnancy and which was found to relax the pelvic ligament in guinea pigs almost a century ago. The broad distribution of relaxin-3 fibres/RXFP3 within several key neuronal circuits suggests an ability to modulate a broad range of behaviours. This has been confirmed in a growing number of rodent studies, which demonstrate relaxin-3 is able to modulate arousal, the response to stress, feeding/metabolism and memory; and likely plays a role in the generation/regulation of hippocampal theta rhythm.

NCBI and Uniprot Product Information

• NCBI GI #: 909618058
• NCBI GeneID: 117579
• NCBI Accession #: NP_001298126.1
• NCBI GenBank Nucleotide #: NM_001311197.1
• UniProt Accession #: Q8WXF3; Q6UXW5
• Molecular Weight: 15,451 Da
• NCBI Official Full Name: relaxin-3
• NCBI Official Synonym Full Names: relaxin 3
• NCBI Official Symbol: RLN3
• NCBI Official Synonym Symbols: H3; RXN3; ZINS4; insl7
• NCBI Protein Information: relaxin-3
• UniProt Protein Name: Relaxin-3
• Protein Family: Relaxin
• UniProt Gene Name: RLN3
• UniProt Synonym Gene Names: INSL7; RXN3; ZINS4; Insulin-like peptide 7
• UniProt Entry Name: REL3_HUMAN

NCBI Description

This gene encodes a member of the relaxin family of insulin-like hormones that is expressed predominantly in the brain and plays a role in physiological processes such as stress, memory and appetite regulation. The encoded protein is a precursor that is proteolytically processed to generate a heterodimeric mature form consisting A and B chains interlinked by disulfide bonds. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]

Uniprot Description

RLN3: May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 (GPCR135) and relaxin-3 receptor-2 (GPCR142). Belongs to the insulin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular region

Molecular Function: G-protein-coupled receptor binding; hormone activity; protein binding