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Typical Testing Data/Standard Curve (for reference only)

Human Pancreasin ELISA Kit | PRSS27 elisa kit

Human Pancreasin ELISA Kit

Gene Names
PRSS27; MPN; CAPH2
Reactivity
Human
Synonyms
Pancreasin; Human Pancreasin ELISA Kit; MPN; Serine protease 27; Marapsin; PRSS27 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
The Human Pancreasin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Pancreasin proteins within the range of 125 pg/ml - 8000 pg/ml.
Sequence Length
188
Samples
Cell Culture Supernates, Serum
Sensitivity
<10 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PRSS27 elisa kit
Principle of the Assay: The Human Pancreasin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Pancreasin in Cell Culture Supernates, Serum. This assay employs an antibody specific for Human Pancreasin coated on a 96-well plate. Standards and samples are pipetted into the wells and Pancreasin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Pancreasin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Pancreasin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Pancreasin, also known as PRSS27 or Marapsin, is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. It belongs to the group-1 subfamily of serine proteases. The pancreasin gene is mapped to chromosome 16p13.3. Pancreasin is resistant to inactivation by large molecular mass trypsin inhibitors, but it is sensitive to benzamidine and leupeptin.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,940 Da
NCBI Official Full Name
serine protease 27 isoform 2
NCBI Official Synonym Full Names
protease, serine 27
NCBI Official Symbol
PRSS27
NCBI Official Synonym Symbols
MPN; CAPH2
NCBI Protein Information
serine protease 27
UniProt Protein Name
Serine protease 27
Protein Family
UniProt Gene Name
PRSS27
UniProt Synonym Gene Names
MPN
UniProt Entry Name
PRS27_HUMAN

NCBI Description

This gene is located within a large protease gene cluster on chromosome 16. It belongs to the group-1 subfamily of serine proteases. The encoded protein is a secreted tryptic serine protease and is expressed mainly in the pancreas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

PRSS27: Belongs to the peptidase S1 family

Protein type: EC 3.4.21.-; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16p13.3

Research Articles on PRSS27

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Product Notes

The Human PRSS27 prss27 (Catalog #AAA8248388) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8248388 ELISA Kit recognizes Human PRSS27. It is sometimes possible for the material contained within the vial of "Pancreasin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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