Rabbit anti-Human Nuclear Receptor LXR beta Polyclonal Antibody | anti-NR1H2 antibody

Nuclear Receptor LXR beta, NT (NR1H2, Liver X Receptor beta, LX Receptor beta, LXR beta, LXRB, LXR-b, NER1, NERI, NER-I, Nuclear Orphan Receptor LXR beta, Nuclear Receptor NER, Nuclear Receptor Subfamily 1 Group H Member 2, Oxysterols Receptor LXR beta, R

Cat. Num. AAA6490654

• Gene Names: NR1H2; NER; UNR; LXRB; LXR-b; NER-I; RIP15
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Nuclear Receptor LXR beta; Polyclonal Antibody; NT (NR1H2; Liver X Receptor beta; LX Receptor beta; LXR beta; LXRB; LXR-b; NER1; NERI; NER-I; Nuclear Orphan Receptor LXR beta; Nuclear Receptor NER; Nuclear Receptor Subfamily 1 Group H Member 2; Oxysterols Receptor LXR beta; R; anti-NR1H2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NR1H2.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NR1H2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human NR1H2 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NR1H2 Antibody (N-term) in Ramos, HL-60 cell line lysates (35ug/lane). NR1H2 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-NR1H2 antibody

Antibodies; Receptors

References

1. Petruzzelli, M., et.al., FEBS Lett. 583(8), 1274-1280 (2009). 2. Dahlman, I., et.al., BMC Med. Genet. 10, 27 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 85362735
• NCBI GeneID: 7376
• NCBI Accession #: NP_009052.3
• NCBI GenBank Nucleotide #: NM_007121.5
• UniProt Accession #: P55055; Q12970; Q5I0Y1; A8K490; B4DNM6; E7EWA6
• Molecular Weight: 39,920 Da
• NCBI Official Full Name: oxysterols receptor LXR-beta isoform 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1, group H, member 2
• NCBI Official Symbol: NR1H2
• NCBI Official Synonym Symbols: NER; UNR; LXRB; LXR-b; NER-I; RIP15
• NCBI Protein Information: oxysterols receptor LXR-beta; LX receptor beta; nuclear receptor NER; liver X nuclear receptor beta; nuclear orphan receptor LXR-beta; steroid hormone-nuclear receptor NER; ubiquitously-expressed nuclear receptor
• UniProt Protein Name: Oxysterols receptor LXR-beta
• Protein Family: Oxysterols receptor
• UniProt Gene Name: NR1H2
• UniProt Synonym Gene Names: LXRB; NER; UNR
• UniProt Entry Name: NR1H2_HUMAN

NCBI Description

The liver X receptors, LXRA (NR1H3; MIM 602423) and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements (summary by Korf et al., 2009 [PubMed 19436111]).[supplied by OMIM, Jan 2010]

Uniprot Description

LXR-beta: Orphan receptor. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Forms a heterodimer with RXR. Ubiquitous. Belongs to the nuclear hormone receptor family. NR1 subfamily.

Protein type: Nuclear receptor

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: retinoid X receptor binding; ligand-dependent nuclear receptor activity; protein binding; DNA binding; zinc ion binding; steroid hormone receptor activity; apolipoprotein A-I receptor binding; ATPase binding

Biological Process: negative regulation of lipid transport; negative regulation of proteolysis; retinoic acid receptor signaling pathway; positive regulation of fatty acid biosynthetic process; transcription initiation from RNA polymerase II promoter; positive regulation of cellular protein metabolic process; cellular lipid metabolic process; negative regulation of pinocytosis; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cholesterol transport; cholesterol homeostasis; positive regulation of lipoprotein lipase activity; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent