Western Blot (WB)
(Western blot analysis of CIDEB expression in HT29 (A), RAW264.7 (B), mouse spleen (C), rat spleen (D) whole cell lysates.)
Rabbit CIDEB Polyclonal Antibody | anti-CIDEB antibody
Anti-CIDEB Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by affinity chromatography.
Synonyms
CIDEB; Polyclonal Antibody; Anti-CIDEB Antibody; Cell death activator CIDE-B; Cell death-inducing DFFA-like effector B; anti-CIDEB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of CIDEB protein.
Purity/Purification
The antibody was purified by affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
219
Applicable Applications for anti-CIDEB antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1/500 - 1/2000; Immunohistochemistry: 1/50 - 1/200; Immunofluorescence/Immunocytochemistry: 1/50 - 1/100
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CIDEB. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of CIDEB expression in HT29 (A), RAW264.7 (B), mouse spleen (C), rat spleen (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of CIDEB expression in HT29 (A), RAW264.7 (B), mouse spleen (C), rat spleen (D) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of CIDEB staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of CIDEB staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of CIDEB staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of CIDEB staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Related Product Information for anti-CIDEB antibody
Rabbit polyclonal antibody to CIDEB
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,678 Da
NCBI Official Full Name
cell death activator CIDE-B
NCBI Official Synonym Full Names
cell death-inducing DFFA-like effector b
NCBI Official Symbol
CIDEB
NCBI Protein Information
cell death activator CIDE-B
UniProt Protein Name
Cell death activator CIDE-B
Protein Family
UniProt Gene Name
CIDEB
UniProt Entry Name
CIDEB_HUMAN
Uniprot Description
Activates apoptosis.
Research Articles on CIDEB
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Product Notes
The CIDEB cideb (Catalog #AAA8248129) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CIDEB Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CIDEB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; Immunohistochemistry: 1/50 - 1/200; Immunofluorescence/Immunocytochemistry: 1/50 - 1/100. Researchers should empirically determine the suitability of the CIDEB cideb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CIDEB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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