Western Blot (WB)
(Western blot analysis of Int11 expression in SKOV3 (A), MCF7 (B), mouse spleen (C), rat liver (D) whole cell lysates.)
Rabbit anti-Human Int11 Polyclonal Antibody | anti-CPSF3L antibody
Anti-Int11 Antibody
Western Blot (WB)
(Western blot analysis of Int11 expression in SKOV3 (A), MCF7 (B), mouse spleen (C), rat liver (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Int11 expression in SKOV3 (A), MCF7 (B), mouse spleen (C), rat liver (D) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Int11 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Int11 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
The Integrator complex contains at least 12 subunits and associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates the 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690). INTS11, or CPSF3L, is the catalytic subunit of the Integrator complex (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM, Mar 2008]
Uniprot Description
INTS11: Catalytic component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage. Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS11 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.27.-; RNA processing; Ribonuclease
Chromosomal Location of Human Ortholog: 1p36.33
Cellular Component: cytoplasm; integrator complex; nucleoplasm
Molecular Function: hydrolase activity; protein binding
Biological Process: snRNA processing; snRNA transcription from RNA polymerase II promoter
Research Articles on CPSF3L
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Product Notes
The CPSF3L cpsf3l (Catalog #AAA8245428) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Int11 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Int11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IF/ICC: 1/10 - 1/100. Researchers should empirically determine the suitability of the CPSF3L cpsf3l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Int11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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