Western Blot (WB)
(Western blot analysis of Exportin 5 expression in MCF7 (A), HIH3T3 (B), PC12 (C), mouse brain (D), rat brain (E) whole cell lysates.)
Rabbit Exportin 5 Polyclonal Antibody | anti-XPO5 antibody
Anti-Exportin 5 Antibody
Western Blot (WB)
(Western blot analysis of Exportin 5 expression in MCF7 (A), HIH3T3 (B), PC12 (C), mouse brain (D), rat brain (E) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Exportin 5 expression in MCF7 (A), HIH3T3 (B), PC12 (C), mouse brain (D), rat brain (E) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Exportin 5 staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Exportin 5 staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Exportin 5 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Exportin 5 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. The encoded protein translocates cargo through the nuclear pore complex in a RanGTP-dependent process. [provided by RefSeq, Aug 2011]
Uniprot Description
Exportin-5: mediates the nuclear export of proteins bearing double-stranded RNA binding domains and double-stranded RNA. Overexpression enhances RNA interference mediated by shRNAs and miRNAs. In the nucleus it binds cooperatively to its cargo and to Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. Exp5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. Two isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; Nuclear export; Nuclear import; Karyopherin
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus
Molecular Function: mRNA binding; protein binding; protein transporter activity; Ran GTPase binding; RNA binding; tRNA binding
Biological Process: pre-microRNA export from nucleus; protein export from nucleus; RNA-mediated gene silencing
Research Articles on XPO5
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Product Notes
The XPO5 xpo5 (Catalog #AAA8245321) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Exportin 5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Exportin 5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IHC: 1/50 - 1/200; IF/ICC: 1/50 - 1/200. Researchers should empirically determine the suitability of the XPO5 xpo5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Exportin 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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