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Typical Testing Data/Standard Curve

Human Surfactant Protein D ELISA Kit | SFTPD elisa kit

Human Surfactant Protein D ELISA Kit

Gene Names
SFTPD; SP-D; PSP-D; SFTP4; COLEC7
Reactivity
Human
Applications
ELISA
Synonyms
Surfactant Protein D; Human Surfactant Protein D ELISA Kit; COLEC7; PSPD; SFTP4; Pulmonary surfactant-associated protein D; PSP-D; SP-D; Collectin-7; Lung surfactant protein D; SFTPD elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
The Human Surfactant Protein D ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Surfactant Protein D proteins within the range of 625 pg/ml - 40000 pg/ml.
Sequence Length
375
Applicable Applications for SFTPD elisa kit
Sandwich ELISA (EIA)
Samples
Cell Culture Supernatants, Serum.
Sensitivity
20 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Typical Testing Data/Standard Curve
Related Product Information for SFTPD elisa kit
Principle of the Assay||The Human Surfactant Protein D ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Surfactant Protein D in Cell Culture Supernatants, Serum. This assay employs an antibody specific for Human Surfactant Protein D coated on a 96-well plate. Standards and samples are pipetted into the wells and Surfactant Protein D present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Surfactant Protein D antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Surfactant Protein D bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What's more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
37,728 Da
NCBI Official Full Name
Pulmonary surfactant-associated protein D
NCBI Official Synonym Full Names
surfactant protein D
NCBI Official Symbol
SFTPD
NCBI Official Synonym Symbols
SP-D; PSP-D; SFTP4; COLEC7
NCBI Protein Information
pulmonary surfactant-associated protein D
UniProt Protein Name
Pulmonary surfactant-associated protein D
UniProt Gene Name
SFTPD
UniProt Synonym Gene Names
COLEC7; PSPD; SFTP4; PSP-D; SP-D
UniProt Entry Name
SFTPD_HUMAN

NCBI Description

The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism. [provided by RefSeq, Jul 2015]

Uniprot Description

SFTPD: Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. Belongs to the SFTPD family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q22.2-q23.1

Cellular Component: clathrin-coated endocytic vesicle; collagen; endocytic vesicle; endoplasmic reticulum membrane; extracellular region; extracellular space; lamellar body; lysosome; proteinaceous extracellular matrix

Molecular Function: carbohydrate binding; protein binding

Biological Process: alveolus development; cellular protein metabolic process; defense response to bacterium; innate immune response; macrophage chemotaxis; negative regulation of interleukin-2 biosynthetic process; negative regulation of T cell proliferation; positive regulation of phagocytosis; receptor-mediated endocytosis; regulation of cytokine production; regulation of immune response; respiratory gaseous exchange; surfactant homeostasis

Research Articles on SFTPD

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Product Notes

The Human SFTPD sftpd (Catalog #AAA8244613) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8244613 ELISA Kit recognizes Human SFTPD. AAA Biotech's Surfactant Protein D can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). Researchers should empirically determine the suitability of the SFTPD sftpd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Surfactant Protein D, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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