Western Blot (WB)
(Western blot analysis of RPS6KC1 expression in Hela (A), HEK293T (B) whole cell lysates.)
Rabbit RPS6KC1 Polyclonal Antibody | anti-RPS6KC1 antibody
Anti-RPS6KC1 Antibody
Gene Names
RPS6KC1; RSKL1; RPK118; S6PKh1; humS6PKh1; S6K-delta-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by affinity chromatography.
Synonyms
RPS6KC1; Polyclonal Antibody; Anti-RPS6KC1 Antibody; RPK118; Ribosomal protein S6 kinase delta-1; S6K-delta-1; 52 kDa ribosomal protein S6 kinase; Ribosomal S6 kinase-like protein with two PSK domains 118 kDa protein; SPHK1-binding protein; anti-RPS6KC1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of RPS6KC1 protein.
Purity/Purification
The antibody was purified by affinity chromatography.
Form/Format
Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% Sodium Azide.
Sequence Length
1066
Applicable Applications for anti-RPS6KC1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
Western Blot: (1/500 - 1/1000); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RPS6KC1. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of RPS6KC1 expression in Hela (A), HEK293T (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of RPS6KC1 expression in Hela (A), HEK293T (B) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of RPS6KC1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of RPS6KC1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Related Product Information for anti-RPS6KC1 antibody
Rabbit polyclonal antibody to RPS6KC1
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
117,273 Da
NCBI Official Full Name
Ribosomal protein S6 kinase delta-1
NCBI Official Synonym Full Names
ribosomal protein S6 kinase C1
NCBI Official Symbol
RPS6KC1
NCBI Official Synonym Symbols
RSKL1; RPK118; S6PKh1; humS6PKh1; S6K-delta-1
NCBI Protein Information
ribosomal protein S6 kinase delta-1
UniProt Protein Name
Ribosomal protein S6 kinase delta-1
Protein Family
UniProt Gene Name
RPS6KC1
UniProt Synonym Gene Names
RPK118; S6K-delta-1
UniProt Entry Name
KS6C1_HUMAN
Uniprot Description
RSKL1: an AGC kinase of the RSKL family.
Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, AGC; AGC group; RSKL family
Chromosomal Location of Human Ortholog: 1q41
Cellular Component: cytoplasm; early endosome; intracellular membrane-bound organelle; membrane
Molecular Function: ATP binding; phosphoinositide binding; protein binding; protein serine/threonine kinase activity
Biological Process: protein amino acid phosphorylation; signal transduction
Research Articles on RPS6KC1
Similar Products
Product Notes
The RPS6KC1 rps6kc1 (Catalog #AAA8243017) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RPS6KC1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RPS6KC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: (1/500 - 1/1000); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500). Researchers should empirically determine the suitability of the RPS6KC1 rps6kc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS6KC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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