Rabbit SDF-1 ALPHA Polyclonal Antibody | anti-SDF-1 ALPHA antibody

RABBIT ANTI HUMAN SDF-1 ALPHA:Biotin

Cat. Num. AAA220218

• Gene Names: CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
• Applications: ELISA, Western Blot
• Synonyms: SDF-1 ALPHA; Polyclonal Antibody; RABBIT ANTI HUMAN SDF-1 ALPHA:Biotin; anti-SDF-1 ALPHA antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Biotin; Purified IgG conjugated to Biotin - lyophilised
• Concentration: IgG concentration 0.1 mg/ml after reconstitution (varies by lot)
• Sequence Length: 93

• Applicable Applications for anti-SDF-1 ALPHA antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: This biotinylated human SDF-1alpha antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human SDF-1alpha antibody as the capture reagent and recombinant human SDF-1alpha as the standard.; Western Blot: This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human SDF-1alpha as the positive control.; ELISA: Minimum Dilution: 0.25; Maximum Dilution: 1.0ug/ml; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antisera to human SDF-1 alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Formalin fixed, paraffin embedded human colorectal carcinoma stained with Rabbit anti Human SDF-1α (MBS221732) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Testing Data #2

(Formalin fixed, paraffin embedded human colorectal carcinoma stained with Rabbit anti Human SDF-1α (MBS221732) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Related Product Information for anti-SDF-1 ALPHA antibody

Rabbit anti Human SDF-1 alpha antibody recognizes human SDF-1 alpha, otherwise known as CXCL12a, a 68 amino acid stromal cell derived CXC chemokine, which arises from alternative splicing of the SDF-1 gene. The two isoforms, SDF-1 alpha and SDF-1 beta, share an identical amino acid sequence, except for an additional four residues in the C-Terminal region of the beta isoform. SDF-1 binds with high-affinity to the G protein -coupled receptor CXCR4 (fusin) and acts as a chemoattractant for T and B lymphocytes, monocytes and migratory neurons, and is a vital factor in haematopoiesis and angiogenesis. By competitively binding to CXCR4, SDF-1 acts as an inhibitor for the CXCR4-mediated entry of HIV-1 virus into target T-cells, and the SDF-1-CXCR4 interaction is important for the regulation of trafficking of normal and malignant stem cells.

NCBI and Uniprot Product Information

• NCBI GI #: 10834988
• NCBI GeneID: 6387
• NCBI Accession #: NP_000600.1
• NCBI GenBank Nucleotide #: NM_000609.6
• UniProt Accession #: P48061; Q2L985; Q2L986; Q2L988; Q5IT36; Q6ICW0; Q9H554; B2R4G0; E7EVL0; H7BYN8
• Molecular Weight: 11,004 Da
• NCBI Official Full Name: stromal cell-derived factor 1 isoform beta
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 12
• NCBI Official Symbol: CXCL12
• NCBI Official Synonym Symbols: IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
• NCBI Protein Information: stromal cell-derived factor 1; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor
• UniProt Protein Name: Stromal cell-derived factor 1
• UniProt Gene Name: CXCL12
• UniProt Synonym Gene Names: SDF1; SDF1A; SDF1B; SDF-1; hSDF-1; IRH; hIRH; PBSF
• UniProt Entry Name: SDF1_HUMAN

NCBI Description

This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL12: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to another C-X-C chemokine receptor CXCR7, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1- alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3- 67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and CXCR7, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T- cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of CXCR7 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Monomer or homodimer; in equilibrium. Dimer formation is induced by non acidic pH and the presence of multivalent anions, and by binding to CXCR4 or heparin. Monomeric form is required for full chemotactic activity and resistance to ischemia/reperfusion injury, whereas the dimeric form acts as a partial agonist of CXCR4, stimulating Ca2+ mobilization but with no chemotactic activity and instead acts as a selective antagonist that blocks chemotaxis induced by the monomeric form. Interacts with the N- terminus of CXCR7. Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. Belongs to the intercrine alpha (chemokine CxC) family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Motility/polarity/chemotaxis; Chemokine; Cell development/differentiation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q11.1

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: growth factor activity; chemokine receptor binding; chemokine activity; CXCR chemokine receptor binding; receptor binding

Biological Process: response to peptide hormone stimulus; positive regulation of dopamine secretion; positive regulation of cell adhesion; blood circulation; adult locomotory behavior; neuron migration; motor axon guidance; chemotaxis; signal transduction; germ cell development; induction of positive chemotaxis; T cell proliferation; response to radiation; germ cell migration; cell adhesion; organ regeneration; regulation of actin polymerization and/or depolymerization; response to virus; cellular calcium ion homeostasis; patterning of blood vessels; G-protein coupled receptor protein signaling pathway; response to heat; response to mechanical stimulus; ameboidal cell migration; positive regulation of axon extension involved in axon guidance; response to hypoxia; positive regulation of endothelial cell proliferation; immune response; telencephalon cell migration

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To