RAB3A sirna

RAB3A siRNA (Human)

Cat. Num. AAA8240878

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAB3A; RAB3A siRNA (Human); Ras-related protein Rab-3A; RAB3A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RAB3A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 220

• Applicable Applications for RAB3A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RAB3A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAB3A sirna

siRNA to inhibit RAB3A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4506367
• NCBI GeneID: 5864
• NCBI Accession #: NP_002857.1
• NCBI GenBank Nucleotide #: NM_002866.4
• UniProt Accession #: P20336; Q9NYE1; A8K0J4
• Molecular Weight: 24,984 Da
• NCBI Official Full Name: ras-related protein Rab-3A
• NCBI Official Synonym Full Names: RAB3A, member RAS oncogene family
• NCBI Official Symbol: RAB3A
• NCBI Protein Information: ras-related protein Rab-3A
• UniProt Protein Name: Ras-related protein Rab-3A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB3A
• UniProt Entry Name: RAB3A_HUMAN

Uniprot Description

RAB3A: Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. Heterodimer with RIMS2. Part of a ternary complex involving PCLO and EPAC2. Interacts with RPH3A. Interacts with the exocyst complex through SEC15. Binds SYTL4, RIMS1 and RIMS2. Interacts with RAB3IP. Interacts with SGSM1 and SGSM3. Specifically expressed in brain. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric, Rab; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: synaptic vesicle; protein complex; axon; secretory granule membrane; acrosome; plasma membrane; terminal button; cytosol; vesicle; endosome

Molecular Function: protein C-terminus binding; GTPase activity; GTPase binding; protein binding; ATPase activator activity; GDP binding; GTP binding; myosin V binding; ATPase binding

Biological Process: mitochondrion organization and biogenesis; synaptic vesicle maturation; metabolic process; regulation of synaptic vesicle fusion to presynaptic membrane; neurotransmitter secretion; constitutive secretory pathway; protein secretion; regulation of short-term neuronal synaptic plasticity; post-embryonic development; intracellular protein transport; synaptic transmission; respiratory system process; synaptic vesicle exocytosis; axonogenesis; glutamate secretion; positive regulation of ATPase activity; sensory perception of touch; neuromuscular synaptic transmission; maintenance of presynaptic active zone structure; response to electrical stimulus; Rab protein signal transduction; vesicle docking during exocytosis; positive regulation of exocytosis; lung development