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Western Blot (WB) (Western Blot analysis of RAB3A in Human cerebella tissue, Human cerebrum tissue and Mouse brain tissue lysates in order using RAB3A Rabbit pAb at dilution 1/900)

Rabbit RAB3A Polyclonal Antibody | anti-RAB3A antibody

RAB3A Rabbit pAb

Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purified
Synonyms
RAB3A; Polyclonal Antibody; RAB3A Rabbit pAb; member RAS oncogene family; anti-RAB3A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
0.9mg/ml (varies by lot)
Applicable Applications for anti-RAB3A antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Fusion protein of human RAB3A
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of RAB3A in Human cerebella tissue, Human cerebrum tissue and Mouse brain tissue lysates in order using RAB3A Rabbit pAb at dilution 1/900)

Western Blot (WB) (Western Blot analysis of RAB3A in Human cerebella tissue, Human cerebrum tissue and Mouse brain tissue lysates in order using RAB3A Rabbit pAb at dilution 1/900)
Related Product Information for anti-RAB3A antibody
Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane. Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane. Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed. Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells. Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9. Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1. Plays also a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,984 Da
NCBI Official Full Name
ras-related protein Rab-3A
NCBI Official Synonym Full Names
RAB3A, member RAS oncogene family
NCBI Official Symbol
RAB3A
NCBI Protein Information
ras-related protein Rab-3A; RAS-associated protein RAB3A
UniProt Protein Name
Ras-related protein Rab-3A
Protein Family
UniProt Gene Name
RAB3A
UniProt Entry Name
RAB3A_HUMAN

Uniprot Description

RAB3A: Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. Heterodimer with RIMS2. Part of a ternary complex involving PCLO and EPAC2. Interacts with RPH3A. Interacts with the exocyst complex through SEC15. Binds SYTL4, RIMS1 and RIMS2. Interacts with RAB3IP. Interacts with SGSM1 and SGSM3. Specifically expressed in brain. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein; G protein, monomeric, Rab; G protein, monomeric

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: synaptic vesicle; protein complex; secretory granule membrane; axon; acrosome; plasma membrane; terminal button; cytosol; vesicle; endosome

Molecular Function: GTPase activity; protein C-terminus binding; protein binding; GTPase binding; ATPase activator activity; GDP binding; GTP binding; myosin V binding; ATPase binding

Biological Process: mitochondrion organization and biogenesis; synaptic vesicle maturation; metabolic process; neurotransmitter secretion; regulation of synaptic vesicle fusion to presynaptic membrane; constitutive secretory pathway; protein secretion; regulation of short-term neuronal synaptic plasticity; post-embryonic development; respiratory system process; intracellular protein transport; synaptic transmission; synaptic vesicle exocytosis; axonogenesis; glutamate secretion; sensory perception of touch; positive regulation of ATPase activity; neuromuscular synaptic transmission; maintenance of presynaptic active zone structure; response to electrical stimulus; Rab protein signal transduction; vesicle docking during exocytosis; positive regulation of exocytosis; lung development

Research Articles on RAB3A

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Product Notes

The RAB3A rab3a (Catalog #AAA4760130) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAB3A Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAB3A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the RAB3A rab3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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