Rabbit anti-Human Metabotropic Glutamate Receptor 6 Polyclonal Antibody | anti-GRM6 antibody

Metabotropic Glutamate Receptor 6, CT (GPRC1F, mGluR6, GRM6, GPRC1F, MGLUR6) (Biotin)

Cat. Num. AAA6488022

• Gene Names: GRM6; mGlu6; CSNB1B; GPRC1F; MGLUR6
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Metabotropic Glutamate Receptor 6; Polyclonal Antibody; CT (GPRC1F; mGluR6; GRM6; GPRC1F; MGLUR6) (Biotin); anti-GRM6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GPRC1F.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GRM6 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human GPRC1F (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GPRC1F Antibody (P847) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GPRC1F antibody detected the GPRC1F protein (arrow).)

Flow Cytometry (FC/FACS)

(Metabotropic Glutamate Receptor 6 (GPRC1F) Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-GRM6 antibody

Antibodies; G Protein Receptors

References

1. Hashimoto, T., et al., Eur. J. Neurosci. 9(6):1226-1235 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 110611176
• NCBI GeneID: 2916
• NCBI Accession #: NP_000834
• NCBI GenBank Nucleotide #: NM_000843
• UniProt Accession #: O15303
• Molecular Weight: 95,468 Da
• NCBI Official Full Name: metabotropic glutamate receptor 6
• NCBI Official Synonym Full Names: glutamate receptor, metabotropic 6
• NCBI Official Symbol: GRM6
• NCBI Official Synonym Symbols: mGlu6; CSNB1B; GPRC1F; MGLUR6
• NCBI Protein Information: metabotropic glutamate receptor 6
• UniProt Protein Name: Metabotropic glutamate receptor 6
• Protein Family: Metabotropic glutamate receptor
• UniProt Gene Name: GRM6
• UniProt Synonym Gene Names: GPRC1F; MGLUR6; mGluR6
• UniProt Entry Name: GRM6_HUMAN

Uniprot Description

mGluR6: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. Defects in GRM6 are the cause of congenital stationary night blindness type 1B (CSNB1B). This disorder consits of a previously unrecognized, autosomal recessive form of congenital night blindness associated with a negative electroretinogram waveform. Patients are night blind from an early age, and when maximally dark-adapted, they could perceive lights only with an intensity equal to or slightly dimmer than that normally detected by the cone system. ERGs in response to single brief flashes of light have clearly detectable a-waves, which are derived from photoreceptors, and greatly reduced b-waves, which are derived from the second-order inner retinal neurons. ERGs in response to sawtooth flickering light indicate a markedly reduced ON response and a nearly normal OFF response. There is no subjective delay in the perception of suddenly appearing white vs black objects on a gray background. Belongs to the G-protein coupled receptor 3 family.

Protein type: Receptor, GPCR; GPCR, family 3; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: presynaptic membrane; Golgi membrane; endoplasmic reticulum membrane; integral to plasma membrane; dendrite; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; group III metabotropic glutamate receptor activity; protein homodimerization activity; glutamate receptor activity

Biological Process: synaptic transmission; regulation of synaptic transmission, glutamatergic; retina development in camera-type eye; metabotropic glutamate receptor signaling pathway; detection of light stimulus involved in visual perception; locomotory behavior; metabotropic glutamate receptor, adenylate cyclase inhibiting pathway; detection of visible light

Disease: Night Blindness, Congenital Stationary, Type 1b