SHH sirna

SHH siRNA (Mouse)

Cat. Num. AAA8239775

• Gene Names: Shh; Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SHH; SHH siRNA (Mouse); HHG1; Sonic hedgehog protein; HHG-1; SHH sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SHH siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 437

• Applicable Applications for SHH sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SHH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SHH sirna

siRNA to inhibit SHH expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21617861
• NCBI GeneID: 20423
• NCBI Accession #: NP_033196.1
• NCBI GenBank Nucleotide #: NM_009170.3
• UniProt Accession #: Q62226
• Molecular Weight: 47,773 Da
• NCBI Official Full Name: sonic hedgehog protein
• NCBI Official Synonym Full Names: sonic hedgehog
• NCBI Official Symbol: Shh
• NCBI Official Synonym Symbols: Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik
• NCBI Protein Information: sonic hedgehog protein
• UniProt Protein Name: Sonic hedgehog protein
• Protein Family: Sonic hedgehog protein
• UniProt Gene Name: Shh
• UniProt Synonym Gene Names: Hhg1; SHH
• UniProt Entry Name: SHH_MOUSE

Uniprot Description

SHH: Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Interacts with HHATL/GUP1 which negatively regulates HHAT-mediated palmitoylation of the SHH N-terminus. N-product is active as a multimer. Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues. Belongs to the hedgehog family.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; Cell cycle regulation; Cell development/differentiation

Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; transport vesicle; cell surface; membrane; cell soma; endoplasmic reticulum; axon; dendrite; plasma membrane; extracellular region; nucleus; lipid raft

Molecular Function: peptidase activity; laminin-1 binding; protein binding; glycosaminoglycan binding; hydrolase activity; zinc ion binding; metal ion binding; patched binding; calcium ion binding; glycoprotein binding

Biological Process: prostate gland development; skin development; developmental growth; central nervous system development; embryonic skeletal development; positive regulation of transcription, DNA-dependent; mesenchymal cell proliferation; telencephalon regionalization; embryonic foregut morphogenesis; positive regulation of mesenchymal cell proliferation; male genitalia development; oligodendrocyte differentiation; anatomical structure formation; kidney development; neural crest cell migration; inner ear development; embryonic limb morphogenesis; hindbrain development; positive regulation of neuroblast proliferation; tongue morphogenesis; cell fate commitment; camera-type eye development; myotube differentiation; neuron fate commitment; intermediate filament organization; positive regulation of skeletal muscle cell proliferation; osteoblast development; response to ethanol; regulation of gene expression; positive regulation of photoreceptor cell differentiation; positive regulation of cell division; regulation of proteolysis; embryonic organ development; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of protein catabolic process; determination of left/right symmetry; neural tube formation; negative regulation of apoptosis; limb development; embryonic forelimb morphogenesis; axon guidance; granule cell precursor proliferation; spinal cord dorsal/ventral patterning; positive regulation of striated muscle cell differentiation; palate development; thalamus development; negative regulation of transcription from RNA polymerase II promoter; signal transduction; Bergmann glial cell differentiation; negative regulation of T cell proliferation; forebrain development; positive regulation of cell proliferation; pancreas development; thyroid gland development; embryonic morphogenesis; angiogenesis; heart looping; ventral spinal cord interneuron specification; vasculogenesis; negative regulation of cell migration; positive regulation of granule cell precursor proliferation; positive thymic T cell selection; gut mesoderm development; intein-mediated protein splicing; regulation of odontogenesis; androgen metabolic process; spinal cord motor neuron differentiation; pattern specification process; regulation of cell proliferation; odontogenesis of dentine-containing teeth; cell proliferation; stem cell development; negative regulation of cell differentiation; embryonic development; positive regulation of protein import into nucleus; dorsal/ventral pattern formation; ureteric bud branching; hindgut morphogenesis; positive regulation of neuron differentiation; lung development; negative regulation of alpha-beta T cell differentiation; negative regulation of Wnt receptor signaling pathway; anatomical structure development; multicellular organismal development; heart development; T cell differentiation in the thymus; CD4-positive or CD8-positive, alpha-beta T cell lineage commitment; lymphoid progenitor cell differentiation; Wnt receptor signaling pathway through beta-catenin; proteolysis; positive regulation of T cell differentiation in the thymus; anterior/posterior pattern formation; hair follicle development; cell-cell signaling; embryonic digestive tract morphogenesis; midbrain development; ectoderm development; positive regulation of smoothened signaling pathway; positive regulation of oligodendrocyte differentiation; oligodendrocyte development; activation of hh target transcription factor; striated muscle development; digestive tract morphogenesis; endocytosis; positive regulation of skeletal muscle development; negative thymic T cell selection; patterning of blood vessels; branching morphogenesis of a tube; polarity specification of anterior/posterior axis; positive regulation of cell differentiation; metanephros development; tongue development; apoptosis; cell fate specification; embryonic hindlimb morphogenesis; odontogenesis; regulation of transcription, DNA-dependent; vasculature development; male genitalia morphogenesis; cell communication; response to axon injury; positive regulation of Wnt receptor signaling pathway; dorsoventral neural tube patterning; organ formation; smoothened signaling pathway; hair follicle morphogenesis; thymus development; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; respiratory tube development; positive regulation of immature T cell proliferation in the thymus; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; formation of anatomical boundary; myoblast differentiation; limb bud formation; establishment of cell polarity; neuroblast proliferation; blood coagulation; cell development; positive regulation of alpha-beta T cell differentiation