ASH2L sirna

ASH2L siRNA (Mouse)

Cat. Num. AAA8239506

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ASH2L; ASH2L siRNA (Mouse); Set1/Ash2 histone methyltransferase complex subunit ASH2; ASH2-like protein; ASH2L sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ASH2L siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 623

• Applicable Applications for ASH2L sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ASH2L gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ASH2L sirna

siRNA to inhibit ASH2L expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 124248552
• NCBI GeneID: 23808
• NCBI Accession #: NP_035921.2
• NCBI GenBank Nucleotide #: NM_011791.3
• UniProt Accession #: Q91X20; Q3UIF9; Q9Z2X4
• Molecular Weight: 68,250 Da
• NCBI Official Full Name: set1/Ash2 histone methyltransferase complex subunit ASH2 isoform a
• NCBI Official Synonym Full Names: ash2 (absent, small, or homeotic)-like (Drosophila)
• NCBI Official Symbol: Ash2l
• NCBI Protein Information: set1/Ash2 histone methyltransferase complex subunit ASH2
• UniProt Protein Name: Set1/Ash2 histone methyltransferase complex subunit ASH2
• UniProt Gene Name: Ash2l
• UniProt Entry Name: ASH2L_MOUSE

Uniprot Description

ASH2L: Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis. Interacts with HCFC1. Core component of several methyltransferase-containing complexes including MLL1/MLL, ASCOM, MLL2/MLL3 and MLL3/MLL4. Each complex is at least composed of ASH2L, RBBP5, DPY30, WDR5, one or more specific histone methyltransferases (MLL, MLL2, MLL3 and MLL4), and the facultative components C16orf53/PA1, C17orf49, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, KAT8/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with DPY30 and RBBP5; the interaction is direct. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Interacts with SETD1A and SETD1B. Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation; Methyltransferase

Cellular Component: histone methyltransferase complex; nucleus

Molecular Function: protein binding; DNA binding; histone lysine N-methyltransferase activity (H3-K4 specific); metal ion binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; response to estrogen stimulus; chromatin-mediated maintenance of transcription; positive regulation of cell proliferation; histone H3-K4 methylation; positive regulation of transcription from RNA polymerase II promoter; chromatin modification; response to DNA damage stimulus