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GGT1 sirna

GGT1 siRNA (Mouse)

Gene Names
Ggt1; GGT; dwg; Ggtp; CD224; GGT 1; GGT-1
Reactivity
Mouse
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
GGT1; GGT1 siRNA (Mouse); GGT; GGTP; Gamma-glutamyltranspeptidase 1; GGT 1; Gamma-glutamyltransferase 1; Glutathione hydrolase 1; Leukotriene-C4 hydrolase; CD antigen CD224; GGT1 sirna
Ordering
For Research Use Only!
Host
Synthetic
Reactivity
Mouse
Specificity
GGT1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
568
Applicable Applications for GGT1 sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GGT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for GGT1 sirna
siRNA to inhibit GGT1 expression using RNA interference

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61,563 Da
NCBI Official Full Name
gamma-glutamyltranspeptidase 1
NCBI Official Synonym Full Names
gamma-glutamyltransferase 1
NCBI Official Symbol
Ggt1
NCBI Official Synonym Symbols
GGT; dwg; Ggtp; CD224; GGT 1; GGT-1
NCBI Protein Information
gamma-glutamyltranspeptidase 1
UniProt Protein Name
Gamma-glutamyltranspeptidase 1
UniProt Gene Name
Ggt1
UniProt Synonym Gene Names
Ggt; Ggtp; GGT 1
UniProt Entry Name
GGT1_MOUSE

NCBI Description

This gene encodes gamma-glutamyl transpeptidase, a plasmamembrane-associated enzyme that cleaves the peptide bond between gamma-glutamyl and cysteinyl glycine moieties of glutathione. The encoded protein is autocatalytically processed to generate an enzymatically active heterodimer comprised of heavy and light chains. Mice lacking the encoded protein grow slowly, develop cataracts and have elevated levels of glutathione in plasma and urine. Transgenic overexpression of the encoded protein in mice enhances osteoclastic bone resorption. The mutant alleles termed 'Dwarf grey' and 'Dwarf grey Bayer' in mice are associated with deletions in this gene. A gamma-glutamyl transpeptidase paralog is located adjacent to this gene. Alternative splicing results in multiple transcript variants. Additional transcripts using alternate promoters and differing in 5' UTRs have been described. [provided by RefSeq, Apr 2015]

Uniprot Description

GGT1: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Defects in GGT1 are a cause of glutathionuria (GLUTH); also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Other Amino Acids Metabolism - glutathione; Other Amino Acids Metabolism - taurine and hypotaurine; EC 2.3.2.2; Other Amino Acids Metabolism - cyanoamino acid; EC 3.4.19.13; Lipid Metabolism - arachidonic acid; Transferase; Other Amino Acids Metabolism - selenoamino acid; EC 3.4.19.14; Membrane protein, integral

Cellular Component: extracellular space; membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: transferase activity; peptidase activity; hydrolase activity; gamma-glutamyltransferase activity; transferase activity, transferring acyl groups

Biological Process: leukotriene biosynthetic process; peptide modification; glutamate metabolic process; regulation of immune system process; glutathione metabolic process; cysteine biosynthetic process; glutathione catabolic process; regulation of inflammatory response; glutathione biosynthetic process; spermatogenesis; zymogen activation; proteolysis

Research Articles on GGT1

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Product Notes

The GGT1 ggt1 (Catalog #AAA8238566) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The GGT1 siRNA (Mouse) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GGT1 can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the GGT1 ggt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GGT1, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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