Rabbit anti-Mouse, Human GGT1 Polyclonal Antibody | anti-GGT1 antibody

Anti-GGT1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101591

• Gene Names: Ggt1; GGT; dwg; Ggtp; CD224; GGT 1; GGT-1
• Reactivity: Mouse, Human
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: GGT1; Polyclonal Antibody; Anti-GGT1 Antibody; Rabbit Polyclonal; GGT1 Antibody; Rabbit PAb; Antigen Affinity Purified; gamma-glutamyltransferase 1; Anti-CD224 Antibody; Anti-dwg Antibody; Anti-GGT Antibody; Anti-GGT-1 Antibody; Anti-Ggtp Antibody; anti-GGT1 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse GGT1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GGT1 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse GGT1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse GGT1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining GGT1 in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining GGT1 in mouse kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-GGT1 antibody

GGT1 belongs to the gamma-glutamyltransferase protein family. Many members of this family have not yet been fully characterized and some of which may represent pseudogenes. GGT1 is composed of a heavy chain and a light chain. It catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. GGT1 also initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracelular GSH level. As part of the cell antioxidant defense mechanism, GGT1 can be detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. Defects in GGT1 can cause glutathionuria which is known as an autosomal recessive disease.

References

Bulle F, et al. (1987) Assignment of the human gamma-glutamyl transferase gene to the long arm of chromosome 22. Hum Genet. 76(3):283-6.Tate SS, et al. (1988) Renal gamma-glutamyl transpeptidases: structural and immunological studies. Arch Biochem Biophys. 262(2):397-408.Tate SS, et al. (1988) In vitro translation and processing of human hepatoma cell (Hep G2) gamma-glutamyl transpeptidase. Biochem Biophys Res Commun. 154(3):1167-73.

NCBI and Uniprot Product Information

• NCBI GI #: 807201132
• NCBI GeneID: 14598
• NCBI Accession #: NP_001292921.1
• NCBI GenBank Nucleotide #: NM_001305992.1
• UniProt Accession #: Q60928
• Molecular Weight: 61,563 Da
• NCBI Official Full Name: gamma-glutamyltranspeptidase 1
• NCBI Official Synonym Full Names: gamma-glutamyltransferase 1
• NCBI Official Symbol: Ggt1
• NCBI Official Synonym Symbols: GGT; dwg; Ggtp; CD224; GGT 1; GGT-1
• NCBI Protein Information: gamma-glutamyltranspeptidase 1
• UniProt Protein Name: Gamma-glutamyltranspeptidase 1
• Protein Family: Gamma-glutamyltranspeptidase
• UniProt Gene Name: Ggt1
• UniProt Synonym Gene Names: Ggt; Ggtp; GGT 1
• UniProt Entry Name: GGT1_MOUSE

NCBI Description

This gene encodes gamma-glutamyl transpeptidase, a plasmamembrane-associated enzyme that cleaves the peptide bond between gamma-glutamyl and cysteinyl glycine moieties of glutathione. The encoded protein is autocatalytically processed to generate an enzymatically active heterodimer comprised of heavy and light chains. Mice lacking the encoded protein grow slowly, develop cataracts and have elevated levels of glutathione in plasma and urine. Transgenic overexpression of the encoded protein in mice enhances osteoclastic bone resorption. The mutant alleles termed 'Dwarf grey' and 'Dwarf grey Bayer' in mice are associated with deletions in this gene. A gamma-glutamyl transpeptidase paralog is located adjacent to this gene. Alternative splicing results in multiple transcript variants. Additional transcripts using alternate promoters and differing in 5' UTRs have been described. [provided by RefSeq, Apr 2015]

Uniprot Description

GGT1: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Defects in GGT1 are a cause of glutathionuria (GLUTH); also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Other Amino Acids Metabolism - cyanoamino acid; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - glutathione; Membrane protein, integral; Transferase; EC 3.4.19.13; Other Amino Acids Metabolism - selenoamino acid; EC 3.4.19.14; Lipid Metabolism - arachidonic acid; EC 2.3.2.2

Cellular Component: extracellular space; integral to membrane; integral to plasma membrane; membrane; plasma membrane

Molecular Function: gamma-glutamyltransferase activity; hydrolase activity; peptidase activity; transferase activity; transferase activity, transferring acyl groups

Biological Process: cysteine biosynthetic process; glutamate metabolic process; glutathione biosynthetic process; glutathione catabolic process; glutathione metabolic process; leukotriene biosynthetic process; peptide modification; proteolysis; regulation of immune system process; regulation of inflammatory response; spermatogenesis; zymogen activation