DISC1 sirna

DISC1 siRNA (Rat)

Cat. Num. AAA8238365

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DISC1; DISC1 siRNA (Rat); Disrupted in schizophrenia 1 homolog; DISC1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: DISC1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 824

• Applicable Applications for DISC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat DISC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DISC1 sirna

siRNA to inhibit DISC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 30842815
• NCBI GeneID: 307940
• NCBI Accession #: NP_783186.2
• NCBI GenBank Nucleotide #: NM_175596.2
• UniProt Accession #: Q810H6
• Molecular Weight: 90,102 Da
• NCBI Official Full Name: disrupted in schizophrenia 1 homolog
• NCBI Official Synonym Full Names: disrupted in schizophrenia 1
• NCBI Official Symbol: Disc1
• NCBI Protein Information: disrupted in schizophrenia 1 homolog
• UniProt Protein Name: Disrupted in schizophrenia 1 homolog
• Protein Family: Disrupted in schizophrenia
• UniProt Gene Name: Disc1
• UniProt Entry Name: DISC1_RAT

NCBI Description

interacts with a variety of cytoskeletal proteins; truncated mutant form is associated with Schizophrenia [RGD, Feb 2006]

Uniprot Description

Involved in the regulation of multiple aspects of embryonic and adult neurogenesis. Required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. Participates in the Wnt-mediated neural progenitor proliferation as a positive regulator by modulating GSK3B activity and CTNNB1 abundance. Plays a role as a modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation. Inhibits the activation of AKT-mTOR signaling upon interaction with CCDC88A. Regulates the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. Plays a role, together with PCNT, in the microtubule network formation.