FAP sirna

FAP siRNA (Human)

Cat. Num. AAA8238190

• Gene Names: FAP; FAPA; SIMP; DPPIV
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FAP; FAP siRNA (Human); Seprase; 170 kDa melanoma membrane-bound gelatinase; Fibroblast activation protein alpha; Integral membrane serine protease; FAP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FAP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 735

• Applicable Applications for FAP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FAP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FAP sirna

siRNA to inhibit FAP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 620597362
• NCBI GeneID: 2191
• NCBI Accession #: NP_001278736.1
• NCBI GenBank Nucleotide #: NM_001291807.1
• UniProt Accession #: Q12884; O00199; Q53TP5; Q86Z29; Q99998; Q9UID4
• Molecular Weight: 26,954 Da
• NCBI Official Full Name: prolyl endopeptidase FAP isoform 2
• NCBI Official Synonym Full Names: fibroblast activation protein, alpha
• NCBI Official Symbol: FAP
• NCBI Official Synonym Symbols: FAPA; SIMP; DPPIV
• NCBI Protein Information: prolyl endopeptidase FAP
• UniProt Protein Name: Prolyl endopeptidase FAP
• Protein Family: Fap1 adhesin
• UniProt Gene Name: FAP
• UniProt Synonym Gene Names: APCE
• UniProt Entry Name: SEPR_HUMAN

NCBI Description

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

Uniprot Description

FAP: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. Belongs to the peptidase S9B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.26; Protease; EC 3.4.14.5; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q23

Cellular Component: extracellular space; cell surface; focal adhesion; lamellipodium; apical part of cell; cytoplasm; plasma membrane; integral to membrane

Molecular Function: protein dimerization activity; integrin binding; peptidase activity; protein binding; protein homodimerization activity; protease binding; dipeptidyl-peptidase activity; serine-type peptidase activity; metalloendopeptidase activity; serine-type endopeptidase activity; endopeptidase activity

Biological Process: proteolysis involved in cellular protein catabolic process; endothelial cell migration; angiogenesis; cell adhesion; proteolysis; regulation of fibrinolysis