MYO1A sirna

MYO1A siRNA (Human)

Cat. Num. AAA8238144

• Gene Names: MYO1A; BBMI; MIHC; MYHL; DFNA48
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYO1A; MYO1A siRNA (Human); MYHL; Unconventional myosin-Ia; Brush border myosin I; BBM-I; BBMI; Myosin I heavy chain; MIHC; MYO1A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MYO1A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1043

• Applicable Applications for MYO1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MYO1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYO1A sirna

siRNA to inhibit MYO1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 365733629
• NCBI GeneID: 4640
• NCBI Accession #: NP_001242970.1
• NCBI GenBank Nucleotide #: NM_001256041.1
• UniProt Accession #: Q9UBC5; Q9UQD7
• Molecular Weight: 118,401 Da
• NCBI Official Full Name: unconventional myosin-Ia
• NCBI Official Synonym Full Names: myosin IA
• NCBI Official Symbol: MYO1A
• NCBI Official Synonym Symbols: BBMI; MIHC; MYHL; DFNA48
• NCBI Protein Information: unconventional myosin-Ia
• UniProt Protein Name: Unconventional myosin-Ia
• Protein Family: Unconventional myosin
• UniProt Gene Name: MYO1A
• UniProt Synonym Gene Names: MYHL; BBM-I; BBMI; MIHC
• UniProt Entry Name: MYO1A_HUMAN

NCBI Description

This gene encodes a member of the myosin superfamily. The protein represents an unconventional myosin; it should not be confused with the conventional skeletal muscle myosin-1 (MYH1). Unconventional myosins contain the basic domains characteristic of conventional myosins and are further distinguished from class members by their tail domains. They function as actin-based molecular motors. Mutations in this gene have been associated with autosomal dominant deafness. Alternatively spliced variants have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

MYO1A: Involved in directing the movement of organelles along actin filaments (Potential). Defects in MYO1A are the cause of deafness autosomal dominant type 48 (DFNA48). DFNA48 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Protein type: Motility/polarity/chemotaxis; Motor; Actin-binding

Chromosomal Location of Human Ortholog: 12q13-q14

Cellular Component: filamentous actin; cortical actin cytoskeleton; microvillus; basolateral plasma membrane; apical plasma membrane; cytoplasm; basal plasma membrane; myosin complex; brush border; lateral plasma membrane

Molecular Function: calmodulin binding; motor activity; actin binding; ATP binding

Biological Process: microvillus biogenesis; sensory perception of sound; metabolic process; vesicle localization

Disease: Deafness, Autosomal Dominant 48