CETN2 sirna

CETN2 siRNA (Human)

Cat. Num. AAA8237354

• Gene Names: CETN2; CALT; CEN2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CETN2; CETN2 siRNA (Human); CALT; CEN2; Centrin-2; Caltractin isoform 1; CETN2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CETN2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 172

• Applicable Applications for CETN2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CETN2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CETN2 sirna

siRNA to inhibit CETN2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4757902
• NCBI GeneID: 1069
• NCBI Accession #: NP_004335.1
• NCBI GenBank Nucleotide #: NM_004344.1
• UniProt Accession #: P41208; Q53XW1; B2R4T4
• Molecular Weight: 19,738 Da
• NCBI Official Full Name: centrin-2
• NCBI Official Synonym Full Names: centrin, EF-hand protein, 2
• NCBI Official Symbol: CETN2
• NCBI Official Synonym Symbols: CALT; CEN2
• NCBI Protein Information: centrin-2
• UniProt Protein Name: Centrin-2
• Protein Family: Centrin
• UniProt Gene Name: CETN2
• UniProt Synonym Gene Names: CALT; CEN2
• UniProt Entry Name: CETN2_HUMAN

NCBI Description

Caltractin belongs to a family of calcium-binding proteins and is a structural component of the centrosome. The high level of conservation from algae to humans and its association with the centrosome suggested that caltractin plays a fundamental role in the structure and function of the microtubule-organizing center, possibly required for the proper duplication and segregation of the centrosome. [provided by RefSeq, Jul 2008]

Uniprot Description

CETN2: a calcium-binding proteins and is a structural component of the centrosome. Contains 4 EF-hand calcium-binding domains. Highly conserved from algae to humans. Its association with the centrosome suggests that it plays a fundamental role in the structure and function of the microtubule-organizing center, possibly required for the proper duplication and segregation of the centrosome.

Protein type: DNA repair, damage; Cell cycle regulation; Calcium-binding

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: centriole; centrosome; intracellular; cytosol; photoreceptor connecting cilium

Molecular Function: protein binding; microtubule binding; G-protein beta/gamma-subunit binding; calcium ion binding; heterotrimeric G-protein binding

Biological Process: mitosis; cell division; nucleotide-excision repair; organelle organization and biogenesis; spermatogenesis; centriole replication; mitotic cell cycle; G2/M transition of mitotic cell cycle; regulation of cytokinesis