ATMIN sirna
ATMIN siRNA (Human)
Gene Names
ATMIN; ASCIZ; ZNF822
Reactivity
Human
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
ATMIN; ATMIN siRNA (Human); KIAA0431; ZNF822; ATM interactor; ATM/ATR-substrate CHK2-interacting zinc finger protein; ASCIZ; Zinc finger protein 822; ATMIN sirna
Host
Synthetic
Reactivity
Human
Specificity
ATMIN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
667
Applicable Applications for ATMIN sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ATMIN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for ATMIN sirna
siRNA to inhibit ATMIN expression using RNA interference
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,334 Da
NCBI Official Full Name
ATM interactor isoform 2
NCBI Official Synonym Full Names
ATM interactor
NCBI Official Symbol
ATMIN
NCBI Official Synonym Symbols
ASCIZ; ZNF822
NCBI Protein Information
ATM interactor
UniProt Protein Name
ATM interactor
Protein Family
UniProt Gene Name
ATMIN
UniProt Synonym Gene Names
KIAA0431; ZNF822; ASCIZ
UniProt Entry Name
ATMIN_HUMAN
Uniprot Description
ATMIN: Transcription factor. Plays a crucial role in cell survival and RAD51 foci formation in response to methylating DNA damage. Involved in regulating the activity of ATM in the absence of DNA damage. May play a role in stabilizing ATM. Binds to the DYNLL1 promoter and activates its transcription. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: C2H2-type zinc finger protein; DNA repair, damage
Chromosomal Location of Human Ortholog: 16q23.2
Cellular Component: nucleus
Molecular Function: dynein binding; protein binding; metal ion binding
Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; response to DNA damage stimulus
Research Articles on ATMIN
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Product Notes
The ATMIN atmin (Catalog #AAA8237014) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The ATMIN siRNA (Human) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATMIN can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the ATMIN atmin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATMIN, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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