Typical Standard Curve/Testing Data
Rat Creatine phosphokinase ELISA Kit | CPK elisa kit
Rat Creatine phosphokinase ELISA Kit
Reactivity
Rat
Synonyms
Creatine phosphokinase; Rat Creatine phosphokinase ELISA Kit; CPK elisa kit
Reactivity
Rat
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for CPK elisa kit
Intended Uses: This CPK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CPK. This ELISA kit for research use only!
Principle of the Assay: CPK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CPK antibody and an CPK-HRP conjugate. The assay sample and buffer are incubated together with CPK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPK concentration since CPK from samples and CPK-HRP conjugate compete for the anti-CPK antibody binding site. Since the number of sites is limited, as more sites are occupied by CPK from the sample, fewer sites are left to bind CPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPK concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CPK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CPK antibody and an CPK-HRP conjugate. The assay sample and buffer are incubated together with CPK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPK concentration since CPK from samples and CPK-HRP conjugate compete for the anti-CPK antibody binding site. Since the number of sites is limited, as more sites are occupied by CPK from the sample, fewer sites are left to bind CPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPK concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,112 Da
NCBI Official Full Name
creatine kinase M-type
NCBI Official Symbol
CKM
NCBI Protein Information
creatine kinase M-type; M-CK; muscle creatine kinase; creatine kinase M chain
UniProt Protein Name
Creatine kinase M-type
UniProt Gene Name
CKM
UniProt Entry Name
KCRM_RABIT
Uniprot Description
CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.
Protein type: Amino Acid Metabolism - arginine and proline; EC 2.7.3.2; Kinase, other
Cellular Component: cytoplasm
Molecular Function: creatine kinase activity; ATP binding
Biological Process: phosphocreatine biosynthetic process; phosphorylation
Research Articles on CPK
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Product Notes
The Rat CPK ckm (Catalog #AAA744369) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA744369 ELISA Kit recognizes Rat CPK. It is sometimes possible for the material contained within the vial of "Creatine phosphokinase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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