DGAT2 sirna

DGAT2 siRNA (Mouse)

Cat. Num. AAA8236453

• Gene Names: Dgat2; ARAT; DGAT-2; 0610010B06Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DGAT2; DGAT2 siRNA (Mouse); Diacylglycerol O-acyltransferase 2; Acyl-CoA retinol O-fatty-acyltransferase; ARAT; Retinol O-fatty-acyltransferase; Diglyceride acyltransferase 2; DGAT2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DGAT2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 388

• Applicable Applications for DGAT2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DGAT2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DGAT2 sirna

siRNA to inhibit DGAT2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16975490
• NCBI GeneID: 67800
• NCBI Accession #: NP_080660.1
• NCBI GenBank Nucleotide #: NM_026384.3
• UniProt Accession #: Q9DCV3
• Molecular Weight: 43,770 Da
• NCBI Official Full Name: diacylglycerol O-acyltransferase 2
• NCBI Official Synonym Full Names: diacylglycerol O-acyltransferase 2
• NCBI Official Symbol: Dgat2
• NCBI Official Synonym Symbols: ARAT; DGAT-2; 0610010B06Rik
• NCBI Protein Information: diacylglycerol O-acyltransferase 2
• UniProt Protein Name: Diacylglycerol O-acyltransferase 2
• Protein Family: Diacylglycerol O-acyltransferase
• UniProt Gene Name: Dgat2
• UniProt Synonym Gene Names: ARAT; Retinol O-fatty-acyltransferase
• UniProt Entry Name: DGAT2_MOUSE

Uniprot Description

DGAT2: Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides. Functions also as an acyl-CoA retinol acyltransferase (ARAT). Belongs to the diacylglycerol acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.20; EC 2.3.1.76; Transferase; Membrane protein, integral; Membrane protein, multi-pass; Lipid Metabolism - glycerolipid; Cofactor and Vitamin Metabolism - retinol

Cellular Component: membrane; intracellular membrane-bound organelle; mitochondrion; cell; perinuclear region of cytoplasm; endoplasmic reticulum; integral to membrane; integral to endoplasmic reticulum membrane; lipid particle

Molecular Function: 2-acylglycerol O-acyltransferase activity; transferase activity; protein homodimerization activity; transferase activity, transferring acyl groups; transferase activity, transferring groups other than amino-acyl groups; retinol O-fatty-acyltransferase activity; diacylglycerol O-acyltransferase activity

Biological Process: cholesterol homeostasis; sequestering of lipid; glycerol metabolic process; fatty acid homeostasis; triacylglycerol biosynthetic process; lipid metabolic process; diacylglycerol metabolic process